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Arabidopsis thaliana

ABSTRACT: Methylation Profile of postmature-green-stage seed and dry seed from Arabidopsis ddcc mutant

PROVIDER: PRJNA281884 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR1984591.fastq.gz	Fastqsanger.gz
	SRR1984592.fastq.gz	Fastqsanger.gz
	SRR1984593.fastq.gz	Fastqsanger.gz
	SRR1984594.fastq.gz	Fastqsanger.gz
	SRR4253521.fastq.gz	Fastqsanger.gz

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Methylation Profile of postmature-green-stage seed and dry seed from Arabidopsis ddcc mutant

Project description:The role of non-CG methylation in seed development and dormancy remains unknown. There are four genes in charge of non-CG methylation in Arabidopsis: drm1, drm2, cmt2 and cmt3. The majority of non-CG methylation in vegetative tissues, leaf, is gone in homozygous ddcc mutant line (Hume et al., 2014). To uncover the possible role of non-CG DNA methylation in seed development and dormancy, we characterized the methylome of ddcc mutant in Arabidopsis postmature-green-stage seed and dry seed using Illumina sequencing. Meanwhile, vegetative tissue, leaves from 3 week plant with ddcc mutant and from wild-type, and postmature-green-stage seed and dry seed from wild-type plant were used as control.

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Project description:The role of on-CG methylation in seed development and dormancy remains unknown. There are four genes in charge of non-CG methylation in Arabidopsis: drm1, drm2, cmt2 and cmt3. The majority of non-CG methylation in vegetative tissues, leaf, is gone in homozygous ddcc mutant line (Hume et al., 2014). To uncover the possible role of non-CG DNA methylation in seed development and dormancy, we characterized the methylome of ddcc mutant in Arabidopsis dry seed using Illumina sequencing. Meanwhile, vegetative tissue, leaves from 3 week plant with ddcc mutant and from wild type, and dry seed from wild type plant were used as control. Illumina sequencing of bisulfite-converted genomic DNA from dry seed and 3-week-plant leaves of ddcc mutant and wild type.

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Project description:Methylation Changes in Arabidopsis Mature Green Seed Parts

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