Project description:Arabidopsis AP1-GR ap1-1 cal-1 were grown under long-day (16 h light/8 h dark) condition. After bolding (26 DAG), half of the plants were treated with dexamethasone. 31 DAG, the untreated inflorescenes were sampled as t0 and the dexamethasone treated one as t5.
Project description:Examination of Columbia-O wildtype and flk mutant tissues under the following conditions:; 7 day-old long-day grown, collected 1hr after dawn (exp3: GSM26236-GSM26241);; 16 day-old continuous-light-grown (exp2: GSM26230-GSM26235);; 16 day-old long-day grown, collected at 16hrs after dawn (exp1: GSM26224-GSM26229).
Project description:Examination of Columbia-O wildtype and flk mutant tissues under the following conditions: 7 day-old long-day grown, collected 1hr after dawn (exp3: GSM26236-GSM26241); 16 day-old continuous-light-grown (exp2: GSM26230-GSM26235); 16 day-old long-day grown, collected at 16hrs after dawn (exp1: GSM26224-GSM26229). Keywords = Arabidopsis Keywords = flowering Keywords = flk Keywords: other
Project description:Purpose: Circadian clock in plants temporally coordinates biological processes throughout the day synchronizing gene expression with environmental changes. Here, we examined the genome-wide circadian and diurnal control of Arabidopsis transcriptome using high throughout RNA-seq approach. Methods: Transcriptional and posttranscritional profiles were identified and characterized for Arabidopsis seedlings grown under continuous light or long-day condition (16 h light/8 h dark) for one day (each condition has two biological replicates). Results: We show that rhythmic posttranscriptional regulation is also a significant factor for genome-wide profile of circadian plant transcriptome. Two major posttranscriptioal mechanisms alternative splicing (AS) and alternative polyadenylation (APA) show circadian rhythmicity, resulting from the oscillation in the genes invovled in AS and APA. Conclusions: Arabidopsis circadian clock not only controls the transcription of genes, but also affects their posttranscriptional regulation through regulating AS and APA.
Project description:The goal of this project is to compare the primary metabolite profile in different tissue types of the model plant Arabidopsis thaliana. Specifically, plants were grown hydroponically under the long-day (16hr light/day) condition at 21C. Tissue samples, including leaves, inflorescences, and roots were harvest 4 1/2 weeks post sowing. Untargeted primary metabolites profiling was carried out using GCTOF.
Project description:Comparison of protein termini in Arabidopsis thaliana vpe0 quadruple mutant and wildtype seedlings shortly after germination to identify differential processed proteins.