Project description:au14-08_xrn_vcs - seed transcriptome of xrn4-5 and vcs-8 mutants. - Role of RNA degradation in seed dormancy. - Study of the seeds regulation by the RNA-decay. Comparison between Col0 total RNA and mutants xrn4-5 and vcs-8 RNA, affected in the dormancy and in RNA metabolism.
Project description:au14-08_xrn_vcs - seed transcriptome of xrn4-5 and vcs-8 mutants. - Role of RNA degradation in seed dormancy. - Study of the seeds regulation by the RNA-decay. Comparison between Col0 total RNA and mutants xrn4-5 and vcs-8 RNA, affected in the dormancy and in RNA metabolism. 6 dye-swap - gene knock out
Project description:We applied the tiling arrays to study the Arabidopsis whole-genome transcriptome in Arabidopsis fry1-6, xrn2-4, xrn3-3 and xrn4-6 mutants.
Project description:To determine whether the developmental defects of urt1-1 xrn4-3 are linked to the biogenesis of spurious siRNAs, we analyzed small RNA libraries and indeed detected the accumulation of 21 nt siRNAs originating from mRNA loci in urt1-1 xrn4-3.
Project description:To obtain a global view of mRNA uridylation in Arabidopsis, we generated TAIL-seq libraries from WT plants, urt1 and xrn4 single mutants, and urt1 xrn4 double mutant. The TAIL-seq protocol was recently developed to deep-sequence the 3' ends of RNAs (Chang et al., 2014). We generated TAIL-seq libraries from WT plants, urt1 and xrn4 single mutants, and urt1 xrn4 double mutant.
Project description:To obtain a global view of mRNA uridylation in Arabidopsis, we generated TAIL-seq libraries from WT plants, urt1 and xrn4 single mutants, and urt1 xrn4 double mutant. The TAIL-seq protocol was recently developed to deep-sequence the 3' ends of RNAs (Chang et al., 2014).
Project description:Application of Parallel Analysis of RNA Ends (PARE), an RNA degradome approach to identify substrates of Arabidopsis XRN4. The xrn4 mutants overaccumulate decay intermediates from multiple mRNA turnover pathways. This material is based on work supported by the National Science Foundation [under Grant Numbers MCB1021636 and MCB1817764 to P.J.G.]
