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Clostridioides difficile

ABSTRACT: BEI Peptoclostridium difficile genome sequencing and assembly

PROVIDER: PRJNA283119 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR2915710_1.fastq.gz	Fastqsanger.gz
	SRR2915710_2.fastq.gz	Fastqsanger.gz
	SRR2915720_1.fastq.gz	Fastqsanger.gz
	SRR2915720_2.fastq.gz	Fastqsanger.gz
	SRR2915730_1.fastq.gz	Fastqsanger.gz

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Project description:Clostridioides difficile is one of the most common nosocomial pathogens and a global public health threat. Upon colonization of the gastrointestinal tract, C. difficile is exposed to a rapidly changing polymicrobial environment and a dynamic metabolic milieu. Despite the link between the gut microbiota and susceptibility to C. difficile, the impact of synergistic interactions between the microbiota and pathogens on the outcome of infection is largely unknown. Here, we show that microbial cooperation between C. difficile and Enterococcus has a profound impact on the growth, metabolism, and pathogenesis of C. difficile.. Through a process of nutrient restriction and metabolite cross-feeding, E. faecalis shapes the metabolic environment in the gut to enhance C. difficile fitness and increase toxin production. These findings demonstrate that members of the microbiota, such as Enterococcus, have a previously unappreciated impact on C. difficile behavior and virulence.

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Project description:The Clostridioides difficile toxins TcdA and TcdB are responsible for diarrhea and colitis. The aim of this project was to explore the effects of the toxins on epithelial barrier function and the molecular mechanisms for diarrhea and inflammation. RNA-seq of toxin-treated intestinal cell monolayers was performed to describe the C. difficile-mediated effects. mRNA profiles from intestinale epithelial cells were generated by deep sequencing using Illumina NovaSeq 6000. This data provide the basis for subsequent upstream regulator analysis.

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