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To Be a Flower or Fruiting Branch: Insights Revealed by mRNA and Small RNA Transcriptomes from Different Cotton Developmental Stages

ABSTRACT: To Be a Flower or Fruiting Branch: Insights Revealed by mRNA and Small RNA Transcriptomes from Different Cotton Developmental Stages

PROVIDER: PRJNA284336 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR2032440_1.fastq.gz	Fastqsanger.gz
	SRR2032440_2.fastq.gz	Fastqsanger.gz
	SRR2032450_1.fastq.gz	Fastqsanger.gz
	SRR2032450_2.fastq.gz	Fastqsanger.gz
	SRR2032460_1.fastq.gz	Fastqsanger.gz

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To Be a Flower or Fruiting Branch: Insights Revealed by mRNA and Small RNA Transcriptomes from Different Cotton Developmental Stages

Project description:Purpose: found out the regulated genes of nulliplex-branch and its forming molecular mechanism Methods: shoot apical mRNA and miRNA in two nulliplex branch and two normal branch cotton of three development stages were generated by deep sequencing, in triplicate, using Illumina HiSeq 2000. Results: we found 3 825 and 353 specific stage differnent expressed genes in pre-budding stage of island cotton and upland cotton, respectively. In miRNA, we found 16 and 18 specific stage differnent expressed miRNA in pre-budding stageof island cotton and upland cotton, respectively. Conclusions: Our study represents the genes and miRNA control development of lateral branch and regulate flowering time at same times.

2016-03-17 | GSE69025 | GEO

To Be a Flower or Fruiting Branch: Insights Revealed by mRNA and Small RNA Transcriptomes from Different Cotton Developmental Stages

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mRNA Transcriptase’s Analyses That Cotton fruit branch Development Status after silenced GhBRC1 gene byVIGS

Project description:Purpose: found out the regulated genes of nulliplex-branch and its forming molecular mechanism Methods: the GhBRC1 genes of nulliplex branch and short branch cotton are silenced by VIGS, and then the shoot apical mRNA of controls and treated were sequenced, in four repeats, using Illumina HiSeq 2000. Results: we found 3519 and 17 differnent expressed genes in nulliplex-branch and short branch cotton, respectively. Conclusions: Our study represents the genes control development of lateral branch.

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To Be a Flower or Fruiting Branch: Insights Revealed by mRNA and Small RNA Transcriptomes from Different Cotton Developmental Stages.

Project description:The architecture of the cotton plant, including fruit branch formation and flowering pattern, is the most important characteristic that directly influences light exploitation, yield and cost of planting. Nulliplex branch is a useful phenotype to study cotton architecture. We used RNA sequencing to obtain mRNA and miRNA profiles from nulliplex- and normal-branch cotton at three developmental stages. The differentially expressed genes (DEGs) and miRNAs were identified that preferentially/specifically expressed in the pre-squaring stage, which is a key stage controlling the transition from vegetative to reproductive growth. The DEGs identified were primarily enriched in RNA, protein, and signalling categories in Gossypium barbadense and Gossypium hirsutum. Interestingly, during the pre-squaring stage, the DEGs were predominantly enriched in transcription factors in both G. barbadense and G. hirsutum, and these transcription factors were mainly involved in branching and flowering. Related miRNAs were also identified. The results showed that fruit branching in cotton is controlled by molecular pathways similar to those in Arabidopsis and that multiple regulated pathways may affect the development of floral buds. Our study showed that the development of fruit branches is closely related to flowering induction and provides insight into the molecular mechanisms of branch and flower development in cotton.

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Gene expression in Tuber melanosporum fruiting bodies at different developmental stages

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mRNA Transcriptase’s Analyses That Cotton fruit branch Development Status after silenced GhBRC1 gene byVIGS

Project description:mRNA Transcriptase’s Analyses That Cotton fruit branch Development Status after silenced GhBRC1 gene byVIGS

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