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Escherichia coli O127:H6 str. E2348/69

ABSTRACT: Comparative genomics provides insight into the diversity of the attaching and effacing Escherichia coli (AEEC) virulence plasmids

PROVIDER: PRJNA285331 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR2044880_1.fastq.gz	Fastqsanger.gz
	SRR2044880_2.fastq.gz	Fastqsanger.gz
	SRR2044881_1.fastq.gz	Fastqsanger.gz
	SRR2044881_2.fastq.gz	Fastqsanger.gz
	SRR2044882_1.fastq.gz	Fastqsanger.gz

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Complete genome sequence and comparative genome analysis of enteropathogenic Escherichia coli O127:H6 strain E2348/69.

Project description:Enteropathogenic Escherichia coli (EPEC) was the first pathovar of E. coli to be implicated in human disease; however, no EPEC strain has been fully sequenced until now. Strain E2348/69 (serotype O127:H6 belonging to E. coli phylogroup B2) has been used worldwide as a prototype strain to study EPEC biology, genetics, and virulence. Studies of E2348/69 led to the discovery of the locus of enterocyte effacement-encoded type III secretion system (T3SS) and its cognate effectors, which play a vital role in attaching and effacing lesion formation on gut epithelial cells. In this study, we determined the complete genomic sequence of E2348/69 and performed genomic comparisons with other important E. coli strains. We identified 424 E2348/69-specific genes, most of which are carried on mobile genetic elements, and a number of genetic traits specifically conserved in phylogroup B2 strains irrespective of their pathotypes, including the absence of the ETT2-related T3SS, which is present in E. coli strains belonging to all other phylogroups. The genome analysis revealed the entire gene repertoire related to E2348/69 virulence. Interestingly, E2348/69 contains only 21 intact T3SS effector genes, all of which are carried on prophages and integrative elements, compared to over 50 effector genes in enterohemorrhagic E. coli O157. As E2348/69 is the most-studied pathogenic E. coli strain, this study provides a genomic context for the vast amount of existing experimental data. The unexpected simplicity of the E2348/69 T3SS provides the first opportunity to fully dissect the entire virulence strategy of attaching and effacing pathogens in the genomic context.

| S-EPMC2612414 | biostudies-literature

Escherichia coli O127:H6 str. E2348/69

Project description:Enteropathogenic strain

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Escherichia coli O127:H6 str. E2348/69

Project description:Identification of transcriptional pathways associated with enviromental modification

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Project description:Escherichia coli O127:H6 str. E2348/69 substr. UMD753 Genome sequencing and assembly

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Escherichia coli O127:H6 str. E2348/69 substr. CVDNalr

Project description:Escherichia coli O127:H6 str. E2348/69 substr. CVDNalr Genome sequencing and assembly

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RIL-seq of EPEC E2348/69 hfq-flag, in virulence activating and non-activating conditions.

Project description:RIL-seq experiment of EPEC hfq-flag mutant, in activating- conditions:growth on DMEM at 37°C to mid exponential growth phase (e.g., OD600=0.3). In these conditions EPEC strongly expresses its major virulence components, T3SS and BFP, mimicking infection. Non-activating conditions: overnight growth of static culture on LB medium at 37°C where virulence factors are not expressed. RIL-seq experiments are designed to reveal the interactions of sRNA and their targets.

2021-01-29 | E-MTAB-8806 | biostudies-arrayexpress

Control of Virulence Gene Expression in Citrobacter rodentium by Trascription Factor RegA

Project description:Identification of the targets of RegA with and without bicarbonate stimulation by comparing RegA knockout to multicopy RegA transgenics. RegA is an AraC like transcription factor identified in a mutational screen for virulence genes in Citrobacter rodentium, an attaching and effacing pathogen that causes transmissible colonic hyperplasia in mice. This experiment compares the RegA null strain with a multicopy plasmid rescue of this null strain in the presence and absence of bicarbonate with the aim of identifying pathogenesis related genes related to the early and late stages of attachment and effacement. Keywords: genetic modification, transcription factor, induction

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Transcriptomic analysis for Pch/ Ler regulon in enterohaemorrhagic Escherichia coli O157:H7

Project description:Enterohaemorrhagic Escherichia coli (EHEC) is an emerging pathogen that causes diarrhea and heamolytic uremic syndrome. Much of the genomic information that affects virulence is acquired via horizontal transfer. Genes necessary for attaching and effacing lesions are located in the LEE pathogenicity island. LEE gene transcription is positively regulated by Ler, which is also encoded by the LEE, and by Pch regulators, which are encoded at other loci. We identified genes whose transcription profiles were similar to those of the LEE genes, by comparing the effects of altering ler and pch transcript levels. We assigned these genes into two classes, according to their transcription profiles. Keywords: Genetic modification

2008-02-01 | GSE9752 | GEO

RNA-seq of EPEC E2348/69 in virulence activating and non-activating conditions. Comparison of wt and dhfq strains.

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2021-01-29 | E-MTAB-8804 | biostudies-arrayexpress

EPEC-E2348/69-V2

Project description:EPEC E2348/69 variety 2. Wild-type strain.

| PRJEB18716 | ENA