Project description:To identify the potential target genes that were affected by the expression of ErbB4 in mouse embryonic stem cells-derived cardiomyocytes Embryonic Stem Cells-Derived Cardiomyocytes were transfected with si-ErbB4 for 48 hours
Project description:To identify the potential target genes that were affected by the expression of ErbB4 in mouse embryonic stem cells-derived cardiomyocytes
Project description:Genome-wide gene expression analysis at different stages of cardiomyocyte differentiation (undifferentiated mouse embryonic stem cells, neonatal mouse cardiomyocytes and adult mouse cardiomyocytes). Results provide important information on the differential expressed genes between undifferentiated mouse embrionic stem cells (mES) and mouse cardiomyocytes (CM) and also between cardiomyocytes from neonatal (CMp) and adult stages (CMa). This dataset allowed us to compare the expression profile of mES, CMp and CMa with the epigenetic profile of histone methylation generated with ChIP-seq experiments. Total RNA was obtained from biological triplicate of undifferentiated mouse embryonic stem cells (mES), neonatal mouse cardiomyocytes (CMp) and adult mouse cardiomyocytes (CMa)
Project description:Genome-wide gene expression analysis at different stages of cardiomyocyte differentiation (undifferentiated mouse embryonic stem cells, neonatal mouse cardiomyocytes and adult mouse cardiomyocytes). Results provide important information on the differential expressed genes between undifferentiated mouse embrionic stem cells (mES) and mouse cardiomyocytes (CM) and also between cardiomyocytes from neonatal (CMp) and adult stages (CMa). This dataset allowed us to compare the expression profile of mES, CMp and CMa with the epigenetic profile of histone methylation generated with ChIP-seq experiments.
Project description:To identify the miRNA that potentially promote the maturation of Embryonic Stem Cells-Derived Cardiomyocytes, we performed miRNA assay profiling to further know the miRNA expression that differentially expressed after coculture with endothelial cells To further know the miRNA expression that differentially expressed after coculture with endothelial cells, we performed miRNA assay profiling to identify the miRNA that potentially promote the maturation of Embryonic Stem Cells-Derived Cardiomyocytes Embryonic Stem Cells-Derived Cardiomyocytes were re-sorted from coculture system with GFP promoter
Project description:RNA sequencing identifies differentially expressed genes in embryonic cardiomyocytes following knockdown of DNMT3a or DNMT3b expression
