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Anguilla anguilla

ABSTRACT: Changes in ovarian gene expression profiles in maturing European eel (Anguilla anguilla) (responders and non-responders)

PROVIDER: PRJNA288489 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR2081180.fastq.gz	Fastqsanger.gz
	SRR2081171.fastq.gz	Fastqsanger.gz
	SRR2081181.fastq.gz	Fastqsanger.gz
	SRR2081172.fastq.gz	Fastqsanger.gz
	SRR2081182.fastq.gz	Fastqsanger.gz

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Anguilla anguilla

Project description:Changes in ovarian gene expression profiles in maturing European eel (Anguilla anguilla)

| PRJNA288478 | ENA

Changes in ovarian gene expression profiles in maturing European eel (Anguilla anguilla) (responders and non-responders)

Project description:The goal of this study was to gain a better understanding of the genetic background of gonadal maturation of the European eel and to use gene expression profiles to identify predictive markers for broodstock selection that can be measured in blood samples. Three-year-old farmed silver female European eels were injected with 20 mg salmon pituitary extract (SPE) once per week and sampled after 4 weekly hormone injections. Four responders (gonadosomatic index > 1.5) and two non-responders (gonadosomatic index < 1.0) were selected for Illumina RNAseq analysis to identify early markers of responsiveness to gonadotropin treatment. Deep-sequencing transcriptome analysis of ovarian samples derived from four responsive and two non-responsive eels after four weekly injections with gonadotropins.

2015-06-30 | E-GEOD-70382 | biostudies-arrayexpress

Transcription profiling of brain from juvenile yellow and adult sexually mature silver eels, Anguilla anguilla

Project description:We investigated the transition from juvenile yellow to the adult sexually maturing, migrating silver eel (Anguilla anguilla) by examining the hypothesis that: The brain is the central organ for the co-ordination of environmental cues (day length, photoperiod, temperature and environmental salinity) with the anatomical and physiological adaptations which accompany pre-migrational morphogenesis and the osmoregulatory plasticity seen in post-migrational, salinity-adapted fish. We have characertised the mRNA expression profiles for the brains of fresh water, yellow and silver eel using a highly representative brain cDNA microarray. The array comprises 5760 cDNA clones from A.anguilla ranging from 0.5 -10 kb and an estimated redundancy of > 5 %.

2008-03-01 | E-MAXD-30 | biostudies-arrayexpress

Transcription profiling of brain from freshwater and sea water adapted silver eels, Anguilla anguilla

Project description:We investigated salinity adaptation during the migration from freshwater to seawater of European eel (Anguilla anguilla) by examining the hypothesis that: The brain is the central organ for the co-ordination of environmental cues (day length, photoperiod, temperature and environmental salinity) with the anatomical and physiological adaptations which accompany pre-migrational morphogenesis and the osmoregulatory plasticity seen in post-migrational, salinity-adapted fish. We have characertised the mRNA expression profiles for the brains of fresh water and sea water adapted silver eel using a highly representative brain cDNA microarray. The array comprises 5760 cDNA clones from A.anguilla ranging from 0.5 -10 kb and an estimated redundancy of > 5 %.

2008-12-31 | E-MAXD-29 | biostudies-arrayexpress

Anguilla anguilla

Project description:Changes in brain gene expression profiles during sexual maturation of European eel (Anguilla anguilla) males.

| PRJNA241161 | ENA

Changes in ovarian gene expression profiles in maturing European eel (Anguilla anguilla) (responders and non-responders)

2015-06-30 | GSE70382 | GEO

European eel Anguilla anguilla individuals from high (Tiber river, Italy) and low pollution (Bolsena lake, Italy) environments

Project description:An European eel-specific microarray platform was developed to identify genes involved in response to pollutants A comparative analysis of gene expression was conducted between European eel Anguilla anguilla individuals from high (Tiber river, Italy) and low pollution (Bolsena lake, Italy) environments. Gene expression profiling was performed using an European eel-specific oligo-DNA microarray of 14,913 probes based on single-colour detection (Cyanine-3 only). Microarrays were scanned with Agilent scanner G2565BA (barcode on the left, DNA on the back surface, scanned through the glass) at a resolution of 5 microns; all slides were scanned twice at two different sensitivity settings (XDRHi 100% and XDRLo 10%); the scanner software created a unique ID for each pair of XDR scans and saved it to both scan image files. Feature Extraction (FE) 9.5 used XDR ID to link the pairs of scans together automatically when extracting data. The signal left after all the FE processing steps have been completed is ProcessedSignal that contains the Multiplicatively Detrended, Background-Subtracted Signal.

2013-05-22 | E-GEOD-35055 | biostudies-arrayexpress

Changes in ovarian gene expression profiles in maturing European eel (Anguilla anguilla)

Project description:The goal of this study was to gain a better understanding of the genetic background of gonadal maturation of the European eel and to use gene expression profiles to identify predictive markers for broodstock selection that can be measured in blood samples. To find leads for maturation markers we performed a pilot deep-sequencing transcriptome analysis of ovarian tissue derived from a yellow eel, a prepubertal silver eel and a post-spawning matured eel. Among the best leads were two key players in steroidogenesis, namely pP450c17 and liver receptor homolog-1.

2015-06-30 | GSE70381 | GEO

Anguilla anguilla isolate:fAngAng1

Project description:Anguilla anguilla (European eel) genome, fAngAng1, alternate haplotype

| PRJNA561984 | ENA

Anguilla anguilla isolate:fAngAng1

Project description:Anguilla anguilla (European eel) genome, fAngAng1, primary haplotype

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