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Malassezia

ABSTRACT: Malassezia furfur and Malassezia Pachydermatis genome sequencing and assembly

OTHER RELATED OMICS DATASETS IN: PXD004523

PROVIDER: PRJNA288787 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
	SRR2086961_1.fastq.gz	Fastqsanger.gz
	SRR2086961_2.fastq.gz	Fastqsanger.gz
	SRR2087004_1.fastq.gz	Fastqsanger.gz
	SRR2087004_2.fastq.gz	Fastqsanger.gz

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Metabolic reconstruction of lipid metabolism of Malassezia

Project description:Malassezia species are lipophilic and lipid dependent yeasts belonging to the human and animal microbiota. Typically, they are isolated from regions rich in sebaceous glands. They have been associated with dermatological diseases such as seborrheic dermatitis, tinea versicolor, atopic dermatitis, and folliculitis. Genome sequences of Malassezia globosa, Malassezia sympodialis, and Malassezia pachydermatis lack genes related to fatty acid synthesis. Here, lipid synthesis pathways of M. furfur, M. pachydermatis, M. globosa, M. sympodialis and an atypical variant of M. furfur were reconstructed using genome data and Constraints Based Reconstruction and Analysis. The metabolic reconstruction allowed us to predict variation in the fluxes of each reaction over the network to satisfy the biomass objective function. Proteomic profiling improved and validated the models through data integration. Results suggest that several mechanisms including steroid and butanoate metabolism explain the yeast’s growth under different lipid conditions. Flux differences were observed in production of riboflavin in M. furfur and the biosynthesis of glycerolipids in the atypical variant of M. furfur and Malassezia sympodialis.

2017-10-02 | PXD004523 | Pride

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The genomic tandem quadruplication is associated with ketoconazole resistance of Malassezia pachydermatis

Project description:Purpose: Understanding the ketoconazole resistance mechanism in Malassezia pachydermatis Methods: The transcriptome profile of the ketoconazole resistant M. pachydermatis KCTC 27587 cells compared to that of ketoconazole sensitive M. pachydermatis CBS 1879 cells were generated by RNA-Seq using Illumina HiSeq. Results: The expressions of genes in the region of quadruplicated chromosome 4 were up-regulated.

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Project description:Malassezia furfur Genome sequencing and assembly

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Integrative genome annotation of Malassezia sympodialis

Project description:The skin commensal yeast Malassezia is associated with several skin disorders. To establish a reference resource, we sought to determine the complete genome sequence of Malassezia sympodialis and identify its protein-coding genes. A novel genome annotation workflow combining RNA sequencing, proteomics, and manual curation was developed to determine gene structures with high accuracy.

2016-12-20 | PXD003773 | Pride

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