Project description:we performed a comparative proteomics analysis of Korla fragrant pear after inoculation with Alternaria sp., at 0h, 24h, 72h, 120 h using iTRAQ-based quantitative proteomic technique. This study aimed to investigate the protein species expression profiles in response to Alternaria sp., infection, explore the potential molecular mechanisms of Korla fragrant pear response against Alternaria sp., and further obtain a comprehensive understanding of Korla fragrant pear-Alternaria sp interactions. The finding may provide novel clues to Korla fragrant pear resistance to Alternaria sp., and may lay the foundation for an in-depth understanding of the interaction between Korla fragrant pear and Alternaria sp., at the proteome level.
Project description:It was previously shown that interferon defects in latently HIV infected cells make them permissive to MG1 mediated killing. To increase effectiveness of MG1 therapy, we have combined MG1 with the SMAC mimetic birinapant to increase MG1 mediated killing in latently infected J1.1 cells. Increased cell death was observed compared to either treatment alone and was accompanied by increased caspase-3/7 and caspase-1 expression. However, cell death could not be blocked by the pan-caspase inhibitor ZVAD-fmk or the necroptosis inhibitor necrostatin1-s, alone or in combination, indicating that cell death does not occur via a single pathway. Furthermore, it was shown that SMAC mimetic treatment results in decreased transcription of genes that take part in the immune signalling pathway, which is likely one the ways how SMAC mimetic treament senstizes HIV infected cells to MG1 mediated death.