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Drosophila melanogaster

ABSTRACT: Genome-wide maps of KDM5 binding in drosophila adults

OTHER RELATED OMICS DATASETS IN: GSE70591

PROVIDER: PRJNA289118 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR2088910.fastq.gz	Fastqsanger.gz
	SRR2088911.fastq.gz	Fastqsanger.gz
	SRR2088912.fastq.gz	Fastqsanger.gz
	SRR2088904.fastq.gz	Fastqsanger.gz
	SRR2088905.fastq.gz	Fastqsanger.gz

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Genome-wide maps of KDM5 binding in drosophila adults

Project description:To determine which genes affected by loss of KDM5 in adults were direct targets, we carried out KDM5 ChIP-seq analyses. To valide this data, we utilized a previously generated fly strain in which the sole source of KDM5 is from a transgene expressing an HA tagged form of KDM5 expressed under the control of its endogenous promoter. Comparing genome-wide gene expression and KDM5 binding analyses in Drosophila adults, we demonstrate the primary function of KDM5 in adults is to activate gene expression KDM5. To investigate the link between KDM5 and H3K4me3, we carried out anti-H3K4me3 ChIP-seq from wildtype adults . Genome-wide, KDM5 and H3K4me3 peaks showed a similar distribution, with both peaking at the transcription start site (TSS) showed a striking overlap with the presence of H3K4me3. Examination of KDM5 binding and histone H3K4me3 modifications in drosophila adults

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Genome-wide maps of KDM5 binding in drosophila adults

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