Project description:A minimal cell cycle control module is driving the cell cycle in these S. Pombe cells. During the experiment the cells are arrested or released to cycle through the different phases of the cell cycle. Samples were collected at the indicated times points from arrested and cycling cells and expression of genes were measured by microarrays on an Affymetrix platform.
Project description:A minimal cell cycle control module is driving the cell cycle in these S. Pombe cells. During the experiment the cells are arrested or released to cycle through the different phases of the cell cycle.
Project description:Expression profiles of polg mutant cells reveal that many genes encoding proteins involved in cell wall biogenesis and stress response are induced, suggesting that polg mutant cells attempt to maintain growth potential and undergo extensive oxidative metabolism. Conversely, many genes encoding proteins involved in ribosome biogenesis and respiration are repressed, indicating that cells depleted of mtDNA are adapted to grow slowly in absence of mitochondrial function. Keywords: Schizosaccharomyces pombe ployg mutant cells vs wild type cells.
Project description:We use high-density tiling DNA microarray technology to obtain the specific transcriptome-wide response induced by 5-Fluorouracil (5FU) in the eukaryotic model Schizosaccharomyces pombe.
