ABSTRACT: Temperature-dependent regulation of gene expression in Japanese flounder Paralichthys olivaceus treated with Edwardsiella tarda formalin-killed cells (FKC)
Project description:Gene expression profiles by microarray have contributed for a elucidation of an immune-response and a determination of efficiency in vaccination. Recent day, edwardsielosis have caused a fatal damage in the aquaculture of Japanese flounder, Paralichthys olivaceus. However the formalin killed-cell vaccines made from Edwardsiella tarda isolated same fish species were not efficient. Recent our study revealed the mixed FKC vaccine made from the two different type of E. tarda protected Japanese flounder against Edwardsiella tarda infection for long-term. In this study, we analyzed the immune-response of a vaccinated fish kidney using the mixed FKC vaccine against Edwardsiella tarda with an Agilent custom-oligo DNA microarray on 9,573 probes of Japanese flounder. Our study revealed that the mixed FKC vaccine confered a strong immune-response and keeped a efficient for long-term on Japanese flounder.
Project description:In order to identify the genes induced by different bacterial cells, which may contain different types of pathogen associated molecular patterns (PAMPs), high-throughput gene expression analyses using Agilent custom-oligo DNA microarray containingon 9,573 probes constructed for Japanese flounder Paralichthys olivaceus were conducted. A number of genes showed significant changes in mRNA levels. However, there are no significant difference in a manner of the changes among the different bacterial cell treatments. The genes significantly induced by the treatments included well-known immune-related genes such as granulocyte-colony stimulation factor, haptoglobin, hepcidin. The kidney were isolated from the formalin killed cells (FKCs) intraperitoneal injected Japanese flounder, Paralichthys olivaceus using the four formalin-killed cells, Edwardsiella tarda strain 54, Lactococcus garviae strain EH8706, Streptococcus iniae strain 02 and Vibrio anguillarum strain H775-3, respectively. Fishes were administered by an intraperitoneal injection using the 1.0 x 10^7 to 1.0 x 10^8 cells of FKCs. After 6 hours from a injection, fish kidney was isolated. We also isolated phosphate-bufferd seline injected fish kidney as a control. We analyzed a four samples in control. We also analyze a three samples in FKC injected fish (16 hybridization).
Project description:In this study, we applied high-throughput sequencing technology to examine miRNAs in Japanese flounder (Paralichthys olivaceus) infected with Streptococcus iniae at different times.
Project description:Identification of the genes differently expressed after Edwardsiella tarda FKC and Streptococcus iniae FKC treatment in Japanese flounder, Paralichthys olivaceus
