Project description:We established simple synthetic microbial communities in a microcosm model system to determine the mechanisms that underlay cross-feeding in microbial methane-consuming communities. Co-occurring strains from Lake Washington sediment were used that are involved in methane consumption, a methanotroph and two non-methanotrophic methylotrophs.
Project description:Maider J. Echeveste Medrano and colleagues characterized the physiological and metabolic response of freshwater methanotrophic archaea to salt stress. The study performed metaproteomics, gene expression profiles and dedicated metabolomics to identify the pathways involved in the salt stress response and the osmolyte present in anaerobic methanotrophic archaea. Correspondence to Cornelia U. Welte c.welte@science.ru.nl
Project description:Anaerobic methanotrophic archaea are key components of the biological methane filter, but progress in understanding their physiology has been limited by the lack of axenic cultures. This study establishes near-axenic electrode-grown cultures of a freshwater anaerobic methanotroph, proposed as Candidatus Methanoperedens vercellensis, and characterizes its physiology, genome, electron transfer properties, and proteins using shotgun proteomics.