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Paracoccus denitrificans Pd1222

ABSTRACT: Experimental evolution of Paracoccus denitrificans Pd1222

PROVIDER: PRJNA290566 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR2131310_1.fastq.gz	Fastqsanger.gz
	SRR2131310_2.fastq.gz	Fastqsanger.gz
	SRR2131330_1.fastq.gz	Fastqsanger.gz
	SRR2131330_2.fastq.gz	Fastqsanger.gz
	SRR2132460_1.fastq.gz	Fastqsanger.gz

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Project description:The alphaproteobacterium Paracoccus denitrificans Pd1222 has two different, yet functionally redundant acetyl-CoA assimilation routes: the Ethylmalonyl-CoA pathway (EMCP) and the glyoxylate cycle (GC). The EMPC and the GC each tightly control the balance between catabolism and anabolism by shifting flux away from the oxidation of acetyl-CoA in the tricarboxylic acid (TCA) cycle towards biomass formation. RamB (encoded by Pden_1365), a member of the ScfR family, is a transcriptional regulator in P. denitrificans Pd1222 that controls expression of GC. To analyze the role of RamB in the coordinated regulation of metabolic plasticity, we analyzed the global transcriptome of P. denitrificans Pd1222, as well as the ramB-deletion strain during mid-exponential growth phase on defined minimal medium with acetate or succinate as carbon sources.

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