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Homo sapiens

ABSTRACT: RNA-sequencing of tamoxifen resistant LY2 cells transfected with siRNA-HOXC11.

PROVIDER: PRJNA290630 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR2124460.fastq.gz	Fastqsanger.gz
	SRR2124461.fastq.gz	Fastqsanger.gz
	SRR2124462.fastq.gz	Fastqsanger.gz
	SRR2124463.fastq.gz	Fastqsanger.gz
	SRR2124464.fastq.gz	Fastqsanger.gz

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RNA-sequencing of tamoxifen resistant LY2 cells transfected with siRNA-HOXC11.

Project description:To assess the global effects of HOXC11 in endocrine resistant breast cancer cells we performed RNA-seq on LY2 cells which were transfected with either siRNA targeting HOXC11 (siHOXC11) or a scrambled negative control siRNA (scrHOXC11) in the presence of 4-OH-tamoxifen (10-8M). Knockdown was verified by Taq-man qRT-PCR prior to library preparation. RNA (10µg) was extracted using an Oligotex mRNA kit (Qiagen) as per manufacturer’s instructions (n=4). RNA was reverse transcribed followed by mRNA library preparation and sequencing based on a protocol outlined by Wilhelm et al., 2010. Sequencing was performed on an Illumina Genome Analyzer II (GAII) (54 million reads per sample on average).

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