Ziziphus nummularia cultivar:Ziziphus nummularia
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PROVIDER: PRJNA290704 | ENA |
REPOSITORIES: ENA
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Project description:Ziziphus nummularia transcriptome under drought stress
| PRJNA291015 | ENA
Project description:Ziziphus nummularia is well distributed in the Thar desert encompassing India and Pakistan. It survives in regions with an average annual rainfall lesser than 100 mm. Transcriptome profiling was done in Z. nummularia genotype CIAHZN-J under control and drought-stress at -0.3MPa. Among the unique transcripts identified, 283 were found to be downregulated and 554 upregulated in comparison to control. The important down-regulated transcripts identified were: LRR receptor like serine/threonine protein kinases, oligo peptide transporter OPT family, myrcene synthase, LRR protein kinase family isoform1, ATP binding cassette transporter, phytochrome kinase, Pectin estrase inhibitor like-35, auxin efflux facilitator isoform 1, multidrug resistance protein, pectin methyl esterase 3 and ABC transporter B family. Similarly some of the upregulated transcripts are: Raffinose synthase family protein, wall associated receptor kinase like, acyl transferase like protein, cadmium/zinc transporting ATPase 3 like, sucrose synthase 6-like, cytochrome P-450 like, phosphatase 2C family protein and TT12-2 MATE transporter. This study will be very useful to identify novel genes that may be involved in drought tolerance of Z. nummularia.
2021-03-31 | GSE69728 | GEO
Project description:Ziziphus nummularia transcriptome analysis under drought
| PRJNA286286 | ENA
Project description:Ziziphus acidojujuba Transcriptome under NaCl stress
| PRJNA776171 | ENA
Project description:Purpose: Mechanism of gene expression changes in autotetraploid drought resistance variation Methods: Leaves under control and drought treatment of 6h, 12h and 48h were sampled from diploid and the autotetraploid for RNA-seq. Total RNA was extracted from 100mg sample with RNeasy plant mini kit (Omega Biotech, China) and purified with RNase-Free DNase set. Following, cDNA library was constructed and the library concentration and insert size was respectively assessed on the Qubit2.0 (Invitrogen, USA) and Agilent Bioanalyzer 2100 system, and then the effective concentration of the library was accurately quantified through Q-PCR. Prepared high quality libraries were sequenced on Illumina HiSeq X-ten platform to generate paired-end raw reads. qRT–PCR validation was performed using SYBR Green assays. Results: The study findings showed that the autotetraploid sour jujube exhibited a superior drought tolerance and enhanced regrowth potential after dehydration in comparison with the diploid counterpart. The physiological responses gradually triggered important functions in the autotetraploid sour jujube under extreme drought conditions. Furthermore, a comparative transcriptome analysis showed that more differentially expressed genes (DEGs) were detected in autotetraploid after drought stress. Through GO enrichment analysis, many DEGs between the diploid and autotetraploid sour jujube after drought-stress exposure were respectively annotated to the oxidation–reduction process, photosystem, DNA binding transcription factor activity, oxidoreductase activity. Consistently, six reactive oxygen species scavenging-related genes were specifically differentially expressed, positive changes of the genes involved in glutathione metabolism pathways were greater and the lower O2− level and malonaldehyde (MDA) content and higher antioxidant enzymes activity were detected in the autotetraploid under drought-stress conditions. The higher chlorophyll content and differentially enriched genes during photosynthesis suggest that the photosynthetic system in the autotetraploid, having variations in stomatal and cellular characteristics, was enhanced during drought stress. In addition, DEGs in the autotetraploid after stress exposure were significantly enriched in DNA-replication and plant hormone, including auxin, abscisic acid and gibberellin signal-transduction pathways. Under osmotic stress conditions, genes associated with the synthesis and transport of osmotic regulatory substances including anthocyanin biosynthesis were differentially expressed, and the soluble sugar, soluble protein and proline contents were significantly higher in the autotetraploid. Moreover, several genes encoding transcription factors (TFs) including GRAS, Bhlh, MYB, WRKY and NAC were induced specifically or to higher levels in the autotetraploid under drought-stress conditions, and hub genes, LOC107403632, LOC107422279, LOC107434947, LOC107412673 and LOC107432609, related to up-regulated transcription factors in the autotetraploid compared with the diploid were identified. Conclusions: Owing to the whole-genome doubling, many functional genes in the autotetraploid plants were differentially expressed compared with in the diploid during drought stress, resulting in resistance difference.
2024-05-26 | GSE175581 | GEO
Project description:Ziziphus jujuba cultivar:Ziziphus jujuba Raw sequence reads
| PRJNA344806 | ENA