Project description:We investigated the transcriptional response to thiamethoxam in the Bemisia tabaci using Illumina sequencing technology. A total of 1,338 genes were differently expressed in the thiamethoxam-resistant whiteflies.
Project description:To investigated the stage-specific gene expression response to thiamethoxam in the Bemisia tabaci, we have designed the Agilent eArray platform to identify stage-regulated gene expression towards thiamethoxam exposure.
Project description:To investigated the stage-specific gene expression response to thiamethoxam in the Bemisia tabaci, we have designed the Agilent eArray platform to identify stage-regulated gene expression towards thiamethoxam exposure. All the B biotype Bemisia tabaci were maintained on cabbage. Thiamethoxam susceptible (TH-S) was cultured without exposure to any chemical insecticides, Thiamethoxam resistance (TH-R) strain exhibited >70-fold resistance to thiamethoxam in comparision to the TH-S strain. Eggs were incubated in 24hours collected as one sample. The fourth nymphs were collected as another sample. The one-day-old unmated adult females were collected as the third samples. Both of the samples were collected from the TH-R, TH-S strains, respectively
Project description:The saliva from Bemisia tabaci (MED biotype) adults was collected using an artificial feeding system and analyzed using an LC-MS/MS proteomics analysis.
