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Mus musculus

ABSTRACT: Role of polycomb repressive complex 1 in the intestinal homeostasis [RNA-seq 2]

PROVIDER: PRJNA292550 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR2155040.fastq.gz	Fastqsanger.gz
	SRR2155041.fastq.gz	Fastqsanger.gz

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Project description:Polycomb-mediated gene repression plays an important role in adult stem cell maintenance. Direct targets of the Polycomb repressive complex PRC2 in th intestinal epithelium were revealed by performing ChIP-sequencing on crypt samples isolated from wild type murine small intestines. The resulting list of H3K27me3-enriched genes were compared with RNA-sequencing data from wild type and Eed knockout crypts. Crypts were isolated from wild type murine intestinal epithelium and subjected to ChIP using anti-H3K27me3 and anti-H3K27Ac antibodies, after which DNA isolated from extracted immunocomplexes was sequenced.

2016-07-01 | E-GEOD-81577 | biostudies-arrayexpress

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