Thermococcus kodakarensis
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ABSTRACT:
PROVIDER: PRJNA292671 | ENA |
REPOSITORIES: ENA
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Project description:A hyperthermophilic archaeon Thermococcus kodakarensis can grow in a wide range of temperatures from 60°C to 93°C, optimally at 85°C. To screen the genes that being specifically expressed at lowest growth temperature, 60°C, we investigated the transcriptional profilings of T. kodakarensis grown at 60˚C by comparing with those grown at 85°C.
2016-04-01 | GSE71987 | GEO
Project description:A hyperthermophilic archaeon Thermococcus kodakarensis can grow in a wide range of temperatures from 60°C to 93°C, optimally at 85°C. To screen the genes that being specifically expressed at lowest growth temperature, 60°C, we investigated the transcriptional profilings of T. kodakarensis grown at 60ËC by comparing with those grown at 85°C. One-condition experiment, KU216 grown at optimal growth temperature, 85°C vs. KU216 grown at lowest growth temperature, 60°C. Technical replicates: 2 KU216 grown at 60°C, independently measured. One replicate per array.
2016-04-01 | E-GEOD-71987 | biostudies-arrayexpress
Project description:Primary transcriptome map of the hyperthermophilic archaeon Thermococcus kodakarensis
| PRJNA242777 | ENA
Project description:Microarray analysis of deletants of two transcription factor B in the hyperthermophilic archaeon, Thermococcus kodakarensis.
| PRJNA215074 | ENA
Project description:Microarray analysis of the deletion of transcription regulator SurR in the hyperthermophilic archaeon Thermococcus kodakarensis
| PRJNA292662 | ENA
Project description:Thermococcus kodakarensis bisulfite-sequenced RNA
| PRJNA937301 | ENA
Project description:Thermococcus kodakarensis KOD1 Epigenomics
| PRJNA209412 | ENA
Project description:Thermococcus kodakarensis is a thermophilic archaea used in this study to determine how changes in growth temperature affect protein expression. This organism was chosen, in part, due to the simplified proteome and potential protein stability gained when analyzing proteins at room temperature. Two temperatures were used as well as one knockout cell line to determine the differences in protein expression. Specifically, changes in protein expression levels were used in conjunction with other data to determine the changing levels of RNA modification based on expression temperature.
2020-02-17 | PXD014814 | Pride
Project description:The study was designed to test the global impact of negative supercoiling on gene expression in model archaeon T. kodakarensis. Exogenous wild type GyrA or GyrAY119F and wild type GyrB from Thermotoga maritima was expressed under the constitutive PhmtB promoter in T. kodakarensis cells.
2021-08-08 | E-MTAB-10799 | biostudies-arrayexpress
Project description:Background: Prokaryotes have relatively small genomes, densely-packed and apparently dominated by protein-encoding sequences. However, data now generated by high throughput RNA sequencing (RNA-seq) reveal surprisingly more-complex transcriptomes with many previously unrecognized and unanticipated non-coding small and antisense transcripts. To date, such studies have investigated primarily Bacteria. Here, we report the transcripts present in Thermococcus kodakarensis, a model hyperthermophilic Archaeon, synthesized under different growth and metabolic conditions. Results: cDNA libraries, generated from RNA preparations isolated from cells growing in media with sulfur or pyruvate, with sulfur to stationary phase, and growing with pyruvate but with sulfur added 20 min before RNA isolation, have been deep-sequenced. The results identify >2,700 sites of transcription initiation, establish a genome-wide map of transcripts, and consensus sequences for transcription initiation and post-transcription regulatory elements in T. kodakarensis. Primary transcription start sites (TSS) are identified upstream of 1,254 annotated genes, including ~78 % of those predicted by promoter locations, and an additional 644 primary TSS and their promoters have been identified within genes. Most of the mRNAs have a 5'-untranslated region (5'-UTR) between 10 and 50 nt long (median length = 16 nt), ~20 % have 5'-UTRs from 50 to 300 nt long, ~14 % are leaderless with 5'-UTRs ≤8 nt, and ~50% contain a consensus ribosome binding sequence. The results also identify TSS for 1,018 antisense transcripts, most with sequences complementary to either the 5'- or 3'-region of a sense mRNA. The data confirm the presence of transcripts from all three CRISPR loci, the RNase P and 7S RNAs, all tRNAs and rRNAs and 69 snoRNAs predicted to be encoded in the T. kodakarensis genome. Two transcripts, putatively identified as riboswitches, were present in RNA preparations isolated from growing but not from stationary phase cells. The procedure used is designed to identify TSS but, assuming that the number of cDNA reads correlates with transcript abundance, the data obtained also provide a semi-quantitative overview of global operon expression. They document substantial differences in gene expression under different physiological conditions and are consistent with previous observations of substrate-dependent specific gene expression. Many previously unrecognized and unanticipated small RNAs have been identified, some with relative low GC contents (≤50%) and sequences that do not fold readily into base-paired secondary structures, contrary to the classical expectations for non-coding RNAs in a hyperthermophile. Conclusion: We have identified >2,700 TSS that include almost all of the primary sites of transcription initiation upstream of annotated genes, and also many secondary sites, sites within genes and sites resulting in antisense transcripts. The T. kodakarensis genome is small (~2.1 Mbp) and tightly packed with protein-encoding genes, but the results reveal the presence of many non-coding RNAs and predict extensive RNA-based regulation in T. kodakarensis.
2014-08-18 | GSE56262 | GEO