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Saccharomyces cerevisiae

ABSTRACT: Genome-wide expression analysis of yeast with CRISPR-mediated inhibition of GAL10 ncRNA compared to wild-type.

PROVIDER: PRJNA292784 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR2157620_1.fastq.gz	Fastqsanger.gz
	SRR2157620_2.fastq.gz	Fastqsanger.gz
	SRR2157630_1.fastq.gz	Fastqsanger.gz
	SRR2157630_2.fastq.gz	Fastqsanger.gz
	SRR2157640_1.fastq.gz	Fastqsanger.gz

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Genome-wide expression analysis of yeast with CRISPR-mediated inhibition of GAL10 ncRNA compared to wild-type.

Project description:We analyzed the genome-wide expression by RNA-seq of a yeast strain that expresses Cas9d and a guideRNA targeted to the GAL10 locus (called +116), which inhibits GAL10 ncRNA expression from the antisense strand. We compared this strain to a strain expressing a scrambled guideRNA. The goal was to examine the effects of ncRNA inhibition and to examine if CRISPR inhibition of gene expression has off-target effects. We find that CRISPR-mediated inhibtion of GAL10 ncRNA only significantly changes expression of transcripts at the GAL1-10 locus, showing that CRISPR is highly specific, and that GAL10 ncRNA only control genes at the GAL locus.

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