Project description:The goal of this study was to determine the effects of ethylene on Azospirillum brasilense strain Sp7 and to deliniate the signal transduction pathway involved in these responses. One main focus was to determine the role of the ethylene receptor, AzoEtr1, in these responses. Since this is a plant-associated bacterium and plants produce ethylene, we evaluated the role of this pathway in mediating the ability of A. brasilense to associate with plants.
Project description:Plant growth promoting rhizobacterium Azospirillum brasilense Sp7 utilizes fructose efficiently via a fructose phosphotransferase system (Fru-PTS). Its genome encodes two putative Fru-PTS, each consisting of FruB (EIIA), FruK (Pfk) and FruA (EIIBC) proteins. We compared the proteomes of A. brasilense Sp7 grown with malate or fructose as sole carbon source, and observed upregulation of component proteins of Fru-PTS1 and several proteins involved in the biogenesis of the type 6 secretion system (T6SS) only on fructose.
