Project description:This study aims to identify and functionally characterize miRNAs and their target genes in juvenile Nicotiana benthamiana plants using miRNA sequencing (miRNA-seq) and degradome sequencing. miRNA-seq will be employed to profile the miRNA repertoire, while degradome sequencing will be used to identify miRNA-mediated mRNA cleavage sites and validate target genes. The combined approach will elucidate the regulatory roles of miRNAs in the early developmental stages of Nicotiana benthamiana and provide insights into their functional characteristics
Project description:Small RNA expression from Nicotiana benthamiana leaves was profiled with the primary goal of ascertaining microRNA isoform diversity for known, conserved families. A secondary goal was to provide a baseline small RNA expression atlas for this species and tissue.
Project description:This study aims to identify and functionally characterize miRNAs and their target genes in juvenile Nicotiana benthamiana plants using miRNA sequencing (miRNA-seq) and degradome sequencing. miRNA-seq will be employed to profile the miRNA repertoire, while degradome sequencing will be used to identify miRNA-mediated mRNA cleavage sites and validate target genes. The combined approach will elucidate the regulatory roles of miRNAs in the early developmental stages of Nicotiana benthamiana and provide insights into their functional characteristics
Project description:In order to find the differentially expressed RNAs and signaling pathways related to the dwarf phenotype induced by NlCSP11, 35S-NlCSP11, 35S-XopQ and 35S-GFP control were infiltrated into Nicotiana benthamiana. We analyzed the differentially expressed RNAs in the infiltrated leaves (NlCSP11_Z/XopQ_Z) and the upper uninfiltrated leaves (NlCSP11_U/XopQ_U) of N. benthamiana. KEGG annotation revealed a large number of differentially expressed RNAs involved in plant-pathogen interactions, indicating that NlCSP11 resulted in a more strong systemic resistance in N. benthamiana.
Project description:In order to find the miRNAs, target genes and signaling pathways related to the dwarf phenotype induced by NlCSP1, 35S-NlCSP1 and 35S-GFP control were infiltrated into Nicotiana benthamiana. We analyzed the differentially expressed miRNAs in the infiltrated leaves (NlCSP1_Z/GFP_Z) and the upper uninfiltrated leaves (NlCSP1_O/GFP_O) of N. benthamiana. A total of 106 and 97 differentially expressed miRNAs were identified in NlCSP1_Z/GFP_Z and NlCSP1_O/GFP_O, respectively. KEGG annotation revealed a large number of differentially expressed miRNAs involved in plant hormone signal transduction, indicating that NlCSP1 changed the hormone signal pathway in N. benthamiana.
