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Global microRNA expression profiling of mouse livers following ischemia-reperfusion injury at different stages

ABSTRACT: Global microRNA expression profiling of mouse livers following ischemia-reperfusion injury at different stages

PROVIDER: PRJNA293705 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR2180250_1.fastq.gz	Fastqsanger.gz
	SRR2180250_2.fastq.gz	Fastqsanger.gz
	SRR2180245_1.fastq.gz	Fastqsanger.gz
	SRR2180245_2.fastq.gz	Fastqsanger.gz
	SRR2180246_1.fastq.gz	Fastqsanger.gz

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Global microRNA expression profiling of mouse livers following ischemia-reperfusion injury at different stages. [miRNA]

Project description:Hepatic ischemia reperfusion injury is a dynamic process consisting of two stages: ischemia and reperfusion, and triggers a cascade of physiological and biochemical events. Given the important role of microRNAs in regulating gene expression, we analyzed gene expression changes in mouse livers at sham control, ischemia stage, and reperfusion stage. We generated global expression profiles of microRNA and mRNA genes in mouse livers subjected to ischemia reperfusion injury at the three stages, respectively. Comparison analysis showed that reperfusion injury had a distinct expression profile whereas the ischemia sample and the sham control were clustered together. Consistently, there are 69 differentially expressed microRNAs between the reperfusion sample and the sham control whereas 28 differentially expressed microRNAs between the ischemia sample and the sham control. We further identified two modes of microRNA expression changes in ischemia reperfusion injury. Functional analysis of both the differentially expressed microRNAs in the two modes and their target mRNAs revealed that ischemia injury impaired mitochondria function, nutrient consumption, and metabolism process. In contrast, reperfusion injury led to severe tissue inflammation that is predominantly an innate-immune response in the ischemia reperfusion process. Our staged analysis of gene expression profiles provides new insights into regulatory mechanisms of microRNAs in mouse hepatic ischemia reperfusion injury.

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Global microRNA expression profiling of mouse livers following ischemia-reperfusion injury at different stages. [mRNA]

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Project description:6-plex TMT-based quantitative proteomics was used to analysis the proteomic profile in different stages of mouse hepatic ischemia-reperfusion injury.

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