ABSTRACT: Response of peripheral blood mononuclear cells from young (≤ 45 years old) and old (>60 years old) healthy donors to influenza virus derived peptide stimulation.
Project description:CD4 T cell responses are characterized based on a limited number of molecular markers selected from exisiting knowledge. The goal of the experiment was to assess antigenic-peptide specific T-cell responses in vitro without bias using microarrays. PBMCs were isolated from 11 young (⤠45 years old) and old (> 60 years old) healthy donors. The cells were stimulated with peptides derived from influenza virus for 24 hours. Unstimulated cells were cultured without the peptides for 24 hours.
Project description:The aim of the study was to test the hypothesis that peptide array reactivity will reveal patterns that can be associated with poor or effective neutralization response to seasonal influenza vaccine We diluted patient sera 1:80 and incubated dilutions on a streptavidin-coated glass surface that was printed with biotinylated peptides derived from seasonal strains of influenza hemagglutinin included in the 2008/2009 trivalent influenza vaccine In this study, serum from 76 study participants, subsetted into young (?30 years old) and elderly (?60 years old) categories were profiled for IgG/IgM antibody reactivity to influenza hemagglutinin peptudes. Using multivariate linear regression and correcting for multiple hypothesis testing by cross-validation, ee found that patterns of peptide reactivity associated with a poor or effective neutralization response to H1N1 and B strains of influenza.
Project description:RNAsequencing from 20 young healthy male (20-45 years) abdominal subcutaneous adipose tissue before and after 60 days' head-down bed rest.
Project description:Microarrays were performed to assess changes in gene expression following treatment of aged mice with human plasma from donors of different ages (young=19-24 years; old=61-82 years).
Project description:Genome wide DNA methylation profiling of muscle cells extracted from healthy donors at different ages. The Illumina Infinium Human Methylation 450 BeadChip was used to obtain DNA methylation profiles across > 485,000 methylation sites. The muscle cells were extracted from young adults (18-40 years old) and elderly (>70 years old)(n=3 per group, 2 groups : Young and aged adult).
Project description:Genome-wide DNA methylation profiling of liver biopsies collected from 45 healthy liver donors ranging from 13 to 90 years old. The Infinium HumanMethylation450 BeadChip was used to obtain DNA methylation profiles across approximately 470,000 CpGs.
Project description:Skin aging is a process of structural and compositional remolding that can be manifested as wrinkling and sagging. Remarkably, the dermis plays a dominant role in the process of skin aging. Recent studies suggest that microRNAs (miRNAs) may play a role in the regulation of gene expression in organism aging. However, studies about age-related miRNAs in human skin remain limited. In order to obtain an overall view of miRNAs expression in human aged dermis, we have investigated the alteration of microRNAs during aging by examining biopsies of human dermis from 12 young and aged donors, and demonstrated that numerous microRNAs showed significant alteration in dermis tissue. Normal human dermal tissue from 12 consenting individuals. Old group vs young group. Old group: with the age over 60 years old; young group: with the age below 10 years old; each group was constituted of 6 individuals.
Project description:Genome wide DNA methylation profiling of Chinese Han male semen samples. The Illumina Human Methylation 450 Beadchip was used to obtain DNA methylation profiles across approximately 450,000 CpGs. Samples included 3 young males (aged 24, 25 and 25 years old) and 3 aged males (aged 63, 60 and 55 years old).
Project description:This study aimed to identify the human T cell response toward different antigen doses. Fresh isolated CD8+ T cells from peripheral blood mononuclear cells (PBMCs) were stimulated with influenza M1 peptide-loaded autologous monocyte-derived dendritic cells for 2 weeks. A high M1 peptide antigen dose (10uM on moDC, HD1-5) and an optimal antigen dose (10nM on moDC OD1-5) were used. M1-specific CD8 T cells were tetramer sorted at the end of the 2 weeks. RNA was extracted and analyzed for gene expression using Agilent 8X60K gene expression microarrays. 5 independent samples from 3 different HLA A2+ blood donors.
