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DNA damage regulation and its role in drug-related phenotypes in the malaria parasites.

ABSTRACT: DNA damage regulation and its role in drug-related phenotypes in the malaria parasites.

PROVIDER: PRJNA294407 | ENA |

REPOSITORIES: ENA

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DNA damage regulation and its role in drug-related phenotypes in the malaria parasites. [RNA]

Project description:The goal of our study is to characterize the global transcriptional resposne of P.falciparum to DNA damageing agents. Alongside, we stidied the epigenetic regulation of DNA damage resposne in parasite. Further, we studied the mecanism which underlined the ARMD (mutator) phenotype of P. falciparum. In order to elucidate the transcriptiona resposne to DNA damage, P. falciparum 3D7 parasites were treated with 4 perturbating agents for 6 hours. We mapped the global nucleosome occupancy for 3 most responsive histone modifications to DNA damage by chromatin immunoprecipitation coupled with DNA microarray (ChIP-on-chip). In order to identify the transcriptional similarities between artemesinin and MMS, we carried out 6 hours of artemesinin treatment in triplicates and compared with triplicate dataset of MMS.

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DNA damage regulation and its role in drug-related phenotypes in the malaria parasites. [genomic DNA]

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DNA damage regulation and its role in drug-related phenotypes in the malaria parasites.

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2016-06-01 | E-GEOD-72580 | biostudies-arrayexpress

DNA damage regulation and its role in drug-related phenotypes in the malaria parasites.

Project description:This SuperSeries is composed of the SubSeries listed below.

2016-06-01 | GSE72580 | GEO

DNA damage regulation and its role in drug-related phenotypes in the malaria parasites.

Project description:DNA of malaria parasites, Plasmodium falciparum, is subjected to extraordinary high levels of genotoxic insults during its complex life cycle within both the mosquito and human host. Accordingly, most of the components of DNA repair machinery are conserved in the parasite genome. Here, we investigated the genome-wide responses of P. falciparum to DNA damaging agents and provided transcriptional evidence of the existence of the double strand break and excision repair system. We also showed that acetylation at H3K9, H4K8, and H3K56 play a role in the direct and indirect response to DNA damage induced by an alkylating agent, methyl methanesulphonate (MMS). Artemisinin, the first line antimalarial chemotherapeutics elicits a similar response compared to MMS which suggests its activity as a DNA damaging agent. Moreover, in contrast to the wild-type P. falciparum, two strains (Dd2 and W2) previously shown to exhibit a mutator phenotype, fail to induce their DNA repair upon MMS-induced DNA damage. Genome sequencing of the two mutator strains identified point mutations in 18 DNA repair genes which may contribute to this phenomenon.

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