Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

The methylome of Alu repeats in primates

ABSTRACT: The methylome of Alu repeats in primates

PROVIDER: PRJNA295234 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR2324000.fastq.gz	Fastqsanger.gz
	SRR2324001.fastq.gz	Fastqsanger.gz
	SRR2324002.fastq.gz	Fastqsanger.gz
	SRR2324003.fastq.gz	Fastqsanger.gz
	SRR2324004.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 16

Similar Datasets

The methylome of Alu repeats in primates

Project description:Next Generation Sequencing of Unmethylated Alu (NSUMA) interrogation of more than 130,000 individual Alus for differential methylation with concomitant analysis of copy number variations applied to the study of hypomethylation in primates. 3 replicates of Gorilla gorilla, Pan troglodytes, Pongo pygmaeus and Homo sapiens were studied.

2015-09-10 | E-GEOD-72877 | biostudies-arrayexpress

The methylome of Alu repeats in primates

2015-09-10 | GSE72877 | GEO

The methylome of Alu repeats in colon cancer

Project description:The methylome of Alu repeats in colon cancer

| PRJNA294809 | ENA

The methylome of Alu repeats in colon cancer (RNA-Seq)

Project description:Poly-A messenger RNA data for the NSUMA-based assessment of Alu repeats methylation status in colorectal cancer

2016-09-28 | GSE87400 | GEO

Alu repeats blocking and CEBPA overexpression in BLaER1 cells, CEBPA ChIP-Seq

Project description:Weaker CEBPA binding in the human than in the mouse genome is a general trait of the human genome across multiple biological conditions. Alu repeats carry strong CEBPA binding motifs, which compete with regulatory regions for CEBPA binding. To directly test this hypothesis, we attempted to overcome Alu competition by using, first, a CRISPR-dCas9 system in BLaER1 cells (Rapino et al. 2013). By promoting the recruitment of the inactive Cas9 to Alu regions with specific gRNAs targeting Alu repeats containing strong CEBPA motifs we protected them, hampering CEBPA binding to these regions. We tested the effect of two different paired gRNA constructs in two replicates and one control paired gRNA in two replicates. Second, we also further overexpressed CEBPA in human BLaER1 cells to overcome Alu competition. We tested two doses of CEBPA overexpression in two replicates and one control experiment in two replicates.

2023-05-01 | E-MTAB-12597 | biostudies-arrayexpress

The methylome of Alu repeats in colon cancer (NSUMA)

Project description:Next Generation Sequencing of Unmethylated Alu (NSUMA) interrogation of more than 130,000 individual Alus for differential methylation with concomitant analysis of copy number variations applied to the study of hypomethylation in colorectal cancer. normal colon tissues, matched primary tumors, blood and colon derived cell lines

2015-09-08 | E-GEOD-72749 | biostudies-arrayexpress

The methylome of Alu repeats in colon cancer (CGH)

2015-09-08 | GSE72750 | GEO

The methylome of Alu repeats in colon cancer (CGH)

Project description:Next Generation Sequencing of Unmethylated Alu (NSUMA) interrogation of more than 130,000 individual Alus for differential methylation with concomitant analysis of copy number variations applied to the study of hypomethylation in colorectal cancer. normal colon tissues, matched primary tumors, and colon derived cell lines

2015-09-08 | E-GEOD-72750 | biostudies-arrayexpress

The methylome of Alu repeats in colon cancer (NSUMA)

2015-09-08 | GSE72749 | GEO

The methylome of Alu repeats in human stem cells

2017-02-23 | GSE95191 | GEO