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Nitratidesulfovibrio vulgaris str. Hildenborough

ABSTRACT: Global transcriptomic profiling of Desulfovibro vulgaris Hildenborough following transitions between sulfate respiration and syntrophy

PROVIDER: PRJNA296030 | ENA |

REPOSITORIES: ENA

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Nitratidesulfovibrio vulgaris str. Hildenborough

Project description:Function of Periplasmic Hydrogenases in the Sulfate-Reducing Bacterium Desulfovibrio vulgaris Hildenborough

| PRJNA101023 | ENA

Combining metabolic flux analysis with proteomics to shed light on the metabolic flexibility: the case of Desulfovibrio vulgaris Hildenborough

Project description:Desulfovibrio vulgaris Hildenborough is a gram-negative anaerobic bacterium belonging to the sulfate-reducing bacteria, a group of microbes that can perform dissimilatory sulfate reduction coupled to the oxidation of various substrates as carbon and energy sources. In the absence of sulfate, they can also ferment organic acids in syntrophy with methanogens. They exhibit high metabolic diversity switching from one energy mode to another depending on nutrients availability in the environments. Hence, they play a central role in shaping ecosystems. Despite, intensive efforts to study D. vulgaris energy metabolism at the genomic, biochemical and ecological level, bioenergetics in this microorganism remains far from being fully understood. Alternatively, few metabolic models were also proposed to explain D. vulgaris bioenergetics. However, they appeared to be not easily adaptable to various environmental conditions. To lift off these limitations, here we constructed a new transparent and robust metabolic model of D. vulgaris bioenergetics by combining whole-cell proteomic analysis with modeling approaches (Flux Balance Analysis). The iDvu71 model showed over 0.95 correlation with experimental data. Further simulations allowed a detailed description of D. vulgaris metabolism in various conditions of growth. Altogether, the simulations run in this study highlighted the sulfate-to-lactate consumption ratio as a pivotal factor in D. vulgaris energy metabolism. In particular, the impact on the hydrogen/formate balance and biomass synthesis is discussed. Overall, this study provides a novel insight into D. vulgaris metabolic flexibility.

2024-02-01 | PXD046638 | Pride

Nitratidesulfovibrio vulgaris str. Hildenborough

Project description:Different Mutants in Desulfovibrio vulgaris Hildenborough

| PRJNA140109 | ENA

Nitratidesulfovibrio vulgaris str. Hildenborough

Project description:Nitrate Stress in Desulfovibrio vulgaris Hildenborough

| PRJNA129789 | ENA

Nitratidesulfovibrio vulgaris str. Hildenborough

Project description:pH stress in Desulfovibrio vulgaris Hildenborough

| PRJNA101107 | ENA

Nitratidesulfovibrio vulgaris str. Hildenborough

Project description:Peroxide stress in Desulfovibrio vulgaris Hildenborough

| PRJNA126343 | ENA

Global transcriptomic profiling of Desulfovibro vulgaris Hildenborough following transitions between sulfate respiration and syntrophy

Project description:Managing tradeoffs through gene regulation is believed to be critical for the success of a generalist in a fluctuating resource environment. To investigate this hypothesis in depth, we imposed a fluctuating environment that required a representative community of the sulfate-reducing generalist Desulfovibrio vulgaris to manage tradeoffs associated with repeated ecologically-relevant shifts between retaining metabolic independence (active capacity for sulfate respiration) and becoming metabolically specialized to a mutualistic association with the hydrogen consuming Methanococcus maripaludis. Counterintuitively, the community rapidly collapsed and went extinct because extensive gene regulation during shifts drove precipitous decline in intracellular abundance of essential transcripts and proteins. We demonstrate that extensive gene regulation can be catastrophic for a generalist when resources fluctuate rapidly, and that the extinction phenomenon can be prevented by a single regulatory mutation that could then potentially serve as a stepping stone for further adaptive evolution in a variable resource environment. Two strains were profiled at early and mid-log phase of growth during Sulfate Respiration and Syntrophy, and after transition between them. 2 biological replicates, 24 samples

2015-09-17 | E-GEOD-73105 | biostudies-arrayexpress

Nitratidesulfovibrio vulgaris str. Hildenborough

Project description:Hydrogen peroxide Stress in Desulfovibrio vulgaris Hildenborough

| PRJNA126347 | ENA

Nitratidesulfovibrio vulgaris str. Hildenborough

Project description:Co-culture stress in Desulfovibrio vulgaris Hildenborough

| PRJNA117909 | ENA

Nitratidesulfovibrio vulgaris str. Hildenborough

Project description:Salt/NaCl stress in Desulfovibrio vulgaris Hildenborough

| PRJNA111527 | ENA