Project description:Human mesenchymal stem cells are differentiated into osteoblasts in the presence or absence of ApoA-I treatment, the global gene profiles of MSCs with different time-points are compared. Day 0 is primary human MSCs without induction was used the baseline for comparision. We used microarrays to investigate the global gene expression profiles in hBM-MSCs with ApoA-I treatment for 1, 3, 5 days during osteogenesis
Project description:Primary human bone marrow-derived mesenchymal stem cells (MSCs) were treated with recombinant human TGFb1 (10ng/ml) for different time points (1, 3, 7, 14, 24 hours)
Project description:Primary human bone marrow-derived mesenchymal stem cells (MSCs) were treated with recombinant human TNFa (50ng/ml) for different time points (1, 3, 7, 14, 24 hours)
Project description:To investigate the mechanisms underlying chondrocyte differentiation of bone marrow mesenchymal stem cells during 3D culture, we employed whole genome microarray expression profiling as a discovery platform to identify key genes influencing differentiation. By comparing differentially expressed genes at different time points, we identified POU5F1 and several autophagy-related genes as potential key players. By using PCR technology and autophagy-related assays, we confirmed that POU5F1 and autophagy pathways influence the chondrogenic differentiation of bone marrow-derived mesenchymal stem cells.
Project description:<p>Bone regeneration requires spatiotemporal coordination of immune modulation, stem cell recruitment, angiogenesis, and osteogenesis, yet most scaffolds lack sequential control across healing phases. We develop a near-infrared (NIR)-responsive therapeutic platform that integrates clinically available irradiation with an engineered 3D radially aligned nanofiber scaffold functionalized with black phosphorus (BP) and a bone marrow mesenchymal stem cell (BMSC)-targeting aptamer (Apt19S). NIR photothermal stimulation accelerates BP degradation, releasing phosphate ions and activating a heat-shock program to promote macrophage polarization, endogenous MSC homing, neovascularization, and osteogenic differentiation. Metabolomics reveals cooperative regulation of HSP-linked signaling and lipid metabolism. In a rat critical-size calvarial defect, the platform achieves robust bone regeneration without exogenous cells or growth factors. The system is simple, structurally tunable, and shape-customizable, providing a clinically translatable and modular framework for spatiotemporal microenvironment programming in bone and other regenerative settings.</p>
Project description:Mesenchymal stem cells derived from human bone marrow have multi-directional differentiation potential.There are differences in expression of related genes in the process of osteogenesis induction. We used microarrays to detail the global programme of gene expression underlying osteogenesis and identified distinct classes of up-regulated genes or down-regulated genes during this process.
Project description:Mesenchymal stem cells derived from human bone marrow have multi-directional differentiation potential.There are differences in expression of related genes in the process of osteogenesis induction. We used microarrays to detail the global programme of gene expression underlying osteogenesis and identified distinct classes of up-regulated genes or down-regulated genes during this process.
Project description:The bone marrow microenvironment in Large Granular Lymphocyte Leukemia (LGLL) patients has been unexplored for it’s role in the development of cytopenias, which lead to complications resulting in the most prominent causes of morbidity and mortality. We used microarrays on primary mesenchymal stem cell (MSC) cultures isolated from bone marrow aspirates from LGLL patients to identify genetic programs that may lead to the observed profibrotic and extrinsically senescent phenotype. Isolated primary MSC cultures were maintained under reduced oxygen conditions (2%). All cultures displayed trilineage pluripotency (adipogenesis, osteogenesis, and chondrogenesis). For comparison, normal MSC cultures in early passage (p2-3; same number of population doublings as the LGLL MSCs) and later passage (p7-9; same time spent in culture as the LGLL MSCs) are included.
