Project description:Nitrogen (N) and phosphorus (P) are pivotal element for proper plant growth and development. We performed microarray analysis of rice shoot and root after nitrogen deficiency (-N) treatment under phosphorus deficiency (-P) condition to obtain a global view of gene regulations associated with plant response to -N under -P condition.
Project description:Phosphate starvation/sufficient rice seedling, root or shoot Pi-starvation or Pi-sufficient stresses responsible rice genes, including previously unannotated genes were identified by Illumina mRNA-seq technology. 53 million reads from Pi-starvation or Pi-sufficient root or shoot tissues were uniquely mapped to the rice genome, and these included 40574 RAP3 transcripts in root and 39748 RAP3 transcripts in shoot. We compared our mRNA-seq expression data with that from Rice 44K oligomicroarray, and about 95.5% (root) and 95.4% (shoot) transcripts supported by the array were confirmed expression both by the array and by mRNA-seq, Moreover, 11888 (root) and 11098 (shoot) RAP genes which were not supported by array, were evidenced expression with mRNA-seq. Furthermore, we discovered 8590 (root) and 8193 (shoot) previously unannotated transcripts upon Pi-starvation and/or Pi-sufficient.
Project description:Macronutrients are pivotal elements for proper plant growth and development. We performed microarray analysis of rice shoot under nitrogen (N), phosphorus (P), and potassium (K) deficiency conditions to obtain a global view of gene regulations associated with plant response to essential nutrients.
Project description:Macronutrients are pivotal elements for proper plant growth and development. We performed microarray analysis of rice shoot under nitrogen (N), phosphorus (P), and potassium (K) deficiency conditions to obtain a global view of gene regulations associated with plant response to essential nutrients. We performed microarray analysis of rice shoot under N, P and K deficiency conditions (1/4, 1/16 and 1/64 of normal concentration) and control for each nutrient. We collected a total of 36 microarray data corresponding to 12 samples with 3 replicates.
Project description:Macronutrients are pivotal elements for proper plant growth and development. We performed microarray analysis of rice root under nitrogen (N), phosphorus (P), and potassium (K) deficiency conditions to obtain a global view of gene regulations associated with plant response to essential nutrients.
Project description:Transcriptional profiling of MIT knockdown plants. MIT is a mitochondrial Fe transporter essential for rice growth and development. The goal was to determine the effects of MIT on global rice gene expression. Control condition experiment, root or shoot of WT vs. MIT knockdown plant. Two replicates each comparison, including a dye swap.
