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Insect metagenome

ABSTRACT: associated microbiota of the cattle tick, Rhipicephalus microplus, larvae.

PROVIDER: PRJNA298221 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR2681680.fastq.gz	Fastqsanger.gz
	SRR2681092.fastq.gz	Fastqsanger.gz
	SRR2681682.fastq.gz	Fastqsanger.gz
	SRR2681683.fastq.gz	Fastqsanger.gz
	SRR2681684.fastq.gz	Fastqsanger.gz

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Cattle lymph nodes: Control vs. Larva tick-infested; Control vs. Adult tick-infested; Larva tick-infested vs. Adult tick-infested

Project description:Transcriptional profiling of lymph nodes of three cattle breeds (Bonsmara, Brahman, Holstein-Friesian) in response to the cattle tick (Rhipicephalus microplus) larvae and adult infestation. Emphasis is placed on firstly, comparing transcriptional responses within a cattle breed, before and after infestation, and secondly, comparing differentially expressed genes common between the breeds.

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Project description:This experiment was undertaken to document changes in gene expression in the skin of tick-resistant Brahman (Bos indicus) and tick-susceptible Holstein-Friesian (Bos taurus) cattle prior to, and following, infestation with the cattle tick Rhipicephalus (Boophilus) microplus Experiment Overall Design: RNA was extracted from skin samples of tick-naÃ¯ve cattle (animals with no previous R.microplus exposure) and tick-infested cattle after a period of successive, heavy infestations with R. microplus. Skin samples taken from tick-infested animals were taken at sites where tick larvae (approximately 24 h old) were attached to the skin sample. Skin samples were of 8 mm diameter and full skin thickness (approximately 10 mm). RNA samples from 12 animals (3 tick-naive Holstein-Friesian, 3 tick-naive Brahman, 3 tick-infested Holstein-Friesian and 3 tick-infested Brahman) were processed and hybridised to individual slides.

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R. microplus expression profiles of larvae pre-attachment and feeding adult female stages in B. indicus and B. taurus

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