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Bos taurus breed:Holstein

ABSTRACT: Mining of lethal recessive genetic variation in Danish cattle

PROVIDER: PRJNA299394 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR2772320_1.fastq.gz	Fastqsanger.gz
	SRR2772320_2.fastq.gz	Fastqsanger.gz
	SRR2770344_1.fastq.gz	Fastqsanger.gz
	SRR2770344_2.fastq.gz	Fastqsanger.gz
	SRR2772316_1.fastq.gz	Fastqsanger.gz

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Transcription profiling of Bos taurus skin of tick naive and tick-infested Holstein-Friesian and Brahman breeds

Project description:This experiment was undertaken to document changes in gene expression in the skin of tick-resistant Brahman (Bos indicus) and tick-susceptible Holstein-Friesian (Bos taurus) cattle prior to, and following, infestation with the cattle tick Rhipicephalus (Boophilus) microplus Experiment Overall Design: RNA was extracted from skin samples of tick-naÃ¯ve cattle (animals with no previous R.microplus exposure) and tick-infested cattle after a period of successive, heavy infestations with R. microplus. Skin samples taken from tick-infested animals were taken at sites where tick larvae (approximately 24 h old) were attached to the skin sample. Skin samples were of 8 mm diameter and full skin thickness (approximately 10 mm). RNA samples from 12 animals (3 tick-naive Holstein-Friesian, 3 tick-naive Brahman, 3 tick-infested Holstein-Friesian and 3 tick-infested Brahman) were processed and hybridised to individual slides.

2009-04-11 | E-GEOD-15342 | biostudies-arrayexpress

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