Project description:RNA-Seq was used to assess the gene expression profiles of 4 allodiploid embryonic stem cell lines and 4 control embryonic stem cell lines. Moreover, single-cell RNA-Seq was used to quantify the transcriptomes of 87 allodiploid single cells. Comparison of mRNA profiles of 4 allodiploid embryonic stem cell lines to 4 control mouse and rat embryonic stem cell lines using the Illumina HiSeq 2000 platform. Single-cell RNA-Seq was conducted to check the transcriptomes of single allodiploid embryonic and differentiated cells.
Project description:RNA-Seq was used to assess the gene expression profiles of 4 allodiploid embryonic stem cell lines and 4 control embryonic stem cell lines. Moreover, single-cell RNA-Seq was used to quantify the transcriptomes of 87 allodiploid single cells.
Project description:Comparative genomic hybridization (CGH) analysis of androgenetic haploid embryonic stem cells was done with 129/Sv male mouse kidney genomic DNA as reference. The results revealed no consistent copy number variations (CNVs) among the examined cell lines (AH129-5, AH129-N1 and AH129-NC1), except for the global difference on the X chromosome to the male control.
Project description:Comparative genomic hybridization (CGH) analysis of androgenetic haploid embryonic stem cells was done with 129/Sv male mouse kidney genomic DNA as reference. The results revealed no consistent copy number variations (CNVs) among the examined cell lines (AH129-5, AH129-N1 and AH129-NC1), except for the global difference on the X chromosome to the male control. Genomic DNA of FACS sorted G0/G1 stage ahES cells was compared with 129/Sv male mouse kidney genomic DNA reference by the NimbleGen Mouse CGH 3x720K Whole-Genome Tiling Array (Build MM9) [100718_MM9_WG_CGH_HX3]
Project description:Comparative genomic hybridization (CGH) analysis of CiPSCs (clone CiPS-57 and CiPS-58) and OSKM-iPSCs. C57BL/6 mouse embryonic fibroblasts (MEFs) genome DNA as a reference. No consistent copy number variations (CNVs) among CiPSCs and OSKM-iPSCs.