Homo sapiens
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA301627 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:In this study, we investigated amplifications and deletions of 49 gastric cancer cell lines by 244k oligonucleotide-based array comparative genomic hybridization.
2016-12-01 | GSE74839 | GEO
Project description:Regions of recurrent genomic amplification and deletion are frequently observed in primary gastric cancers (GC). However, identifying specific oncogenes and tumor suppressor genes within these regions can be challenging, as they often cover tens to hundreds of genes. Here, we combined high-resolution array-based comparative genomic hybridization (aCGH) with gene expression profiling to target genes lying within focal high-level amplifications in GC cell lines, and identified RAB23 as an amplified and overexpressed Chr 6p11p12 gene in Hs746T cells. High RAB23 protein expression was also observed in some lines lacking RAB23 amplification, suggesting additional mechanisms besides gene amplification for up-regulating RAB23. siRNA silencing of RAB23 reduced the invasive potential of both amplified and nonamplified GC cell lines. RAB23 gene amplifications were observed in 13% of primary gastric carcinomas. In two independent patient cohorts, RAB23 transcript and protein expression was significantly associated with diffuse-type gastric cancer (dGC) compared to intestinal-type gastric cancer (iGC), providing further evidence that dGC and iGC likely represent two molecularly distinct tumor types. Our study demonstrates that investigating focal chromosomal amplifications by combining highresolution aCGH with expression profiling is a powerful general strategy for identifying novel cancer genes in recurrent regions of chromosomal aberration. Keywords: gastric cancer cell lines, comparative genomic hybridization, gene expression profiling
2008-12-29 | GSE10611 | GEO
Project description:Genomic profiling of gastric adenomas by array-based comparative genomic hybridization
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Project description:This SuperSeries is composed of the following subset Series: GSE19566: Genomic profiling of gastric carcinoma in situ by array-based comparative genomic hybridization GSE19574: Genomic profiling of gastric adenomas by array-based comparative genomic hybridization Refer to individual Series
2010-01-31 | E-GEOD-19575 | biostudies-arrayexpress
Project description:Genomic profiling of pancreatic cancer cell lines by array-based comparative genomic hybridization
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Project description:Regions of recurrent genomic amplification and deletion are frequently observed in primary gastric cancers (GC). However, identifying specific oncogenes and tumor suppressor genes within these regions can be challenging, as they often cover tens to hundreds of genes. Here, we combined high-resolution array-based comparative genomic hybridization (aCGH) with gene expression profiling to target genes lying within focal high-level amplifications in GC cell lines, and identified RAB23 as an amplified and overexpressed Chr 6p11p12 gene in Hs746T cells. High RAB23 protein expression was also observed in some lines lacking RAB23 amplification, suggesting additional mechanisms besides gene amplification for up-regulating RAB23. siRNA silencing of RAB23 reduced the invasive potential of both amplified and nonamplified GC cell lines. RAB23 gene amplifications were observed in 13% of primary gastric carcinomas. In two independent patient cohorts, RAB23 transcript and protein expression was significantly associated with diffuse-type gastric cancer (dGC) compared to intestinal-type gastric cancer (iGC), providing further evidence that dGC and iGC likely represent two molecularly distinct tumor types. Our study demonstrates that investigating focal chromosomal amplifications by combining highresolution aCGH with expression profiling is a powerful general strategy for identifying novel cancer genes in recurrent regions of chromosomal aberration. Keywords: gastric cancer cell lines, comparative genomic hybridization, gene expression profiling Affy 100K SNP profiling and 32K BAC Array profiling for 7 Gastric Cancer Cell Lines
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Project description:Genomic copy number aberrations of 11 gastric cancer cell lines were analyzed by 244k CGH array from Agilent Technologies. Based on this results, we separated the 11 cell lines into 2 groups, with and without copy number increase at chromosome 20q13 We performed array comparative genomic hybridization to detect genomic copy number aberrations in 11 gastric cancer cell lines. Microarray images were analyzed by using Feature Extraction and DNA analytics softwares.
2016-04-01 | E-GEOD-67604 | biostudies-arrayexpress
Project description:An oligo array based high-resolution analysis of copy number alterations in 171 primary breast tumors of relatively small size and low NPI, and 49 breast cancer cell-lines. Objectives of the study were to study the molecular taxonomy and the genomic aberration patterns in a breast cancer cohort representative of breast cancer demographics. Keywords: array comparative genomic hybridisation
2007-08-16 | GSE8757 | GEO
Project description:Genomic profiling of gastric carcinoma in situ by array-based comparative genomic hybridization
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Project description:In this study, we investigated CNAs of 59 tumor samples from 27 patients with submucosal-invasive gastric cancers (SMGC) by 44k oligonucleotide-based array comparative genomic hybridization (array CGH).
2012-01-20 | GSE26800 | GEO