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Arabidopsis thaliana

ABSTRACT: RNA-seq analysis of chitin-triggered transcriptional reprogramming in the Arabidopsis mapkkk5 mutant

PROVIDER: PRJNA302003 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR2921850.fastq.gz	Fastqsanger.gz
	SRR2921860.fastq.gz	Fastqsanger.gz
	SRR2921851.fastq.gz	Fastqsanger.gz
	SRR2921861.fastq.gz	Fastqsanger.gz
	SRR2921852.fastq.gz	Fastqsanger.gz

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RNA-seq analysis of chitin-triggered transcriptional reprogramming in the Arabidopsis mapkkk5 mutant

Project description:Perception of microbe-associated molecular patterns by host cell-surface pattern recognition receptors triggers a series of immune responses such as activation of mitogen-activated protein kinase (MAPK) cascades. In Arabidopsis thaliana, the receptor-like cytoplasmic kinase PBL27 interacts with the chitin receptor CERK1 and regulates chitin-induced MAPK activation. We found that the MAPK kinase kinase MAPKKK5 connects PBL27 to the downstream MAPK Kinases (MKK4/5) and MAPKs (MPK3/6). RNA-seq analysis using the mapkkk5 mutant indicated that MAPKKK5 plays important roles in chitin-induced transcriptional reprogramming. As far as we are aware, this is the first report pointing to the significance of a complete MAPK cascade in defense-associated transcriptional reprogramming in plants.

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Transcriptional profiling of an Fd-GOGAT1/GLU1 mutant in Arabidopsis thaliana

Project description:Transcriptional profiling of an Fd-GOGAT1/GLU1 mutant in Arabidopsis thaliana reveals a multiple stress response and extensive reprogramming of the transcriptome Glutamate plays a central position in the synthesis of a variety of organic molecules in plants and is synthesised from nitrate through a series of enzymatic reactions. Glutamate synthases catalyse the last step in this pathway and two types are present in plants: NADH- or ferredoxin-dependent. Here we report a genome wide microarray analysis of the transcriptional reprogramming that occurs in leaves and roots of the A. thaliana mutant glu1-2 knocked-down in the expression of Fd-GOGAT1 (GLU1; At5g04140), one of the two genes of A. thaliana encoding ferredoxin-dependent glutamate synthase. Rosette leaves and roots from the glu1-2 mutant, which is a T-DNA knockout of a ferredoxin dependent glutamate synthase in Arabidopsis (locus ID: At5g04140), were analyzed on Affymetrix microarrays. As a reference control, leaves and roots from Col(0) plants were used. The glu1-2 mutant is derived from a Col(0) accession. Four independent biological replicas from leaves and roots were used in the analysis (from both glu1-2 and Col(0) reference). In total 16 hybridizations were done.

2010-06-08 | E-GEOD-20493 | biostudies-arrayexpress

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