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Fusarium oxysporum f. melongenae

ABSTRACT: rRNA depleted total RNA genome sequencing

PROVIDER: PRJNA303949 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR2960134.fastq.gz	Fastqsanger.gz
	SRR2960134_1.fastq.gz	Fastqsanger.gz
	SRR2960134_2.fastq.gz	Fastqsanger.gz

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Phenotypical and gene expression analyses of Rfo-sa1 resistant eggplant interaction with Fusarium oxysporum f. sp. melongenae and Verticillium dahliae

Project description:Eggplant is susceptible to fungal wilts caused by Fusarium oxysporum f. sp. melongenae and Verticillium dahliae. Wild relatives represent a good source of resistance and ILs have been obtained through introgression of the Rfo-sa1 locus conferring resistance to Fusarium oxysporum from the allied species S. aethiopicum into cultivated eggplant. In this work, a deep phenotypical characterization was performed according to the progression of symptoms along the stem and the disease severity in leaves. This analysis showed that the Fom -resistant ILs carrying introgression of the Rfo-sa1 locus displayed significantly improved tolerance to Verticillium attack after a preliminary inoculation with F. oxysporum. This positive effect was particularly evident when Verticillium inoculation was done simultaneously or after the Fusarium one. Transcript profiling carried out using a combination of SSH, microarray and qRT-PCR analyses from inoculated roots with a selected combinations of fungal pathogens enabled the identification of 164? differentially expressed genes at least in one condition. Overall, our results highlighted a number of candidate genes putatively involved in early defence responses or signalling pathways activated upon infection of eggplant either with Fom and Vd and thus leading to a broad Rfo-sa1 mediated tolerance against both these wilt pathogens.

2017-01-26 | GSE75958 | GEO

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