Pseudomonas aeruginosa AES-1R
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA303970 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR2954855.fastq.gz | Fastqsanger.gz | |||
| SRR2954855_1.fastq.gz | Fastqsanger.gz | |||
| SRR2954855_2.fastq.gz | Fastqsanger.gz | |||
| SRR2954856.fastq.gz | Fastqsanger.gz | |||
| SRR2954856_1.fastq.gz | Fastqsanger.gz |
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Project description:Bacteria in biofilms have higher antibiotic tolerance than their planktonic counterparts. A major outstanding question is the degree to which the biofilm-specific cellular state and its constituent genetic determinants contribute to this hyper-tolerant phenotype. Here, using genome-wide functional profiling of a complex, heterogeneous mutant population of Pseudomonas aeruginosa MPAO1, we identified large sets of mutations that contribute to antibiotic tolerance predominantly in the biofilm or planktonic setting only. Our mixed population-based experimental design recapitulated the complexity of natural biofilms and, unlike previous studies, revealed clinically observed behaviors including the emergence of quorum sensing-deficient mutants. Our study revealed a substantial contribution of the cellular state to the antibiotic tolerance of biofilms, providing a rational foundation for the development of novel therapeutics against P. aeruginosa biofilm-associated infections. This dataset compares the expression of SAH108, a strain with enhanced antibiotic tolerance in the biofilm state, to expression in wild-type strains.
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