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Danio rerio

ABSTRACT: Single epicardial cell transcriptome sequencing identifies Caveolin-1 as an essential factor in zebrafish heart regeneration

PROVIDER: PRJNA304633 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR2968430_1.fastq.gz	Fastqsanger.gz
	SRR2968430_2.fastq.gz	Fastqsanger.gz
	SRR2968440_1.fastq.gz	Fastqsanger.gz
	SRR2968440_2.fastq.gz	Fastqsanger.gz
	SRR2968450_1.fastq.gz	Fastqsanger.gz

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Project description:Telomerase is essential for zebrafish heart regeneration

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Single epicardial cell transcriptome sequencing identifies Caveolin-1 as an essential factor in zebrafish heart regeneration

Project description:By contrast with mammals, adult zebrafish have a high capacity to regenerate damaged or lost myocardium through proliferation of spared cardiomyocytes. The epicardial sheet covering the heart is activated by injury and aids muscle regeneration through paracrine effects and as a multipotent cell source, and has received recent attention as a target in cardiac repair strategies. While it is recognized that epicardium is required for muscle regeneration and itself has high regenerative potential, the extent of cellular heterogeneity within epicardial tissue is largely unexplored. In this study, we performed transcriptome analysis on dozens of epicardial lineage cells purified from zebrafish harboring a transgenic reporter for the pan-epicardial gene tcf21. Hierarchical clustering analysis suggested the presence of at least three epicardial cell subsets defined by expression signatures. We validated many new pan-epicardial and epicardial markers by alternative expression assays. Additionally, we explored the function of the scaffolding protein and main component of caveolae, caveolin-1 (cav1), which was present in each epicardial subset. In BAC transgenic zebrafish, cav1 regulatory sequences drove strong expression in ostensibly all epicardial cells and in coronary vascular endothelial cells. Moreover, cav1 mutant zebrafish generated by genome editing showed grossly normal heart development and adult cardiac anatomy, but displayed profound defects in injury-induced cardiomyocyte proliferation and heart regeneration. Our study defines a new platform for the discovery of epicardial lineage markers, genetic tools, and mechanisms of heart regeneration. Deep sequencing of isolated single epicardial cells

2015-12-15 | E-GEOD-75583 | biostudies-arrayexpress

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Project description:Identification of enhancer regulatory elements that direct epicardial gene expression during zebrafish heart regeneration