Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Yersinia pestis biovar Medievalis str. Harbin 35

ABSTRACT: Virulent human isolate

PROVIDER: PRJNA30505 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Yersinia pestis biovar Microtus str. 91001

Project description:Extremely virulent organism that causes plague

| PRJNA10638 | ENA

Human macrophages exhibit high activity to clear intracellular biovar Microtus strain of Y. pestis

Project description:human peripheral lymphocytes were infected with the full virulent strain 141 or human avirulent Microtus strain 201, and their transcriptomes were determined and compared. The most impressive finding is that robust responses of lysosome, TLR signaling and FcR mediated phagocytosis pathways were provoked at 2 hpi in 201- but not in 141-infected lymphocytes, suggesting that human lymphocytes might be able to constrain infection caused by strain 201 but not 141.

2019-05-15 | GSE121084 | GEO

Identification and characterization of PhoP regulon members in Yersinia pestis biovar Microtus.

Project description:BackgroundThe transcription regulator PhoP has been shown to be important for Y. pestis survival in macrophages and under various in vitro stresses. However, the mechanism by which PhoP promotes bacterial intracellular survival is not fully understood. Our previous microarray analysis suggested that PhoP governed a wide set of cellular pathways in Y. pestis. A series of biochemical experiments were done herein to study members of the PhoP regulon of Y. pestis biovar Microtus.ResultsBy using gel mobility shift assay and quantitative RT-PCR, a total of 30 putative transcription units were characterized as direct PhoP targets. The primer extension assay was further used to determine the transcription start sites of 18 PhoP-dependent promoters and to localize the -10 and -35 elements. The DNase I footprinting was used to identify the PhoP-binding sites within 17 PhoP-dependent promoters, enabling the identification of PhoP box and matrix that both represented the conserved signals for PhoP recognition in Y. pestis. Data presented here providing a good basis for modeling PhoP-promoter DNA interactions that is crucial to the PhoP-mediated transcriptional regulation.ConclusionThe proven direct PhoP targets include nine genes encoding regulators and 21 genes or operons with functions of detoxification, protection against DNA damages, resistance to antimicrobial peptides, and adaptation to magnesium limitation. We can presume that PhoP is a global regulator that controls a complex regulatory cascade by a mechanism of not only directly controlling the expression of specific genes, but also indirectly regulating various cellular pathways by acting on a set of dedicated regulators. These results help us gain insights into the PhoP-dependent mechanisms by which Y. pestis survives the antibacterial strategies employed by host macrophages.

| S-EPMC2322996 | biostudies-literature

Yersinia pestis biovar Orientalis str. IP674

Project description:Yersinia pestis biovar Orientalis str. IP674 Genome sequencing

| PRJEA42373 | ENA

Yersinia pestis biovar Orientalis str. AS200901509

Project description:Genomic biosurveillance of 2009 epizootic Yersinia pestis in New Mexico, USA

| PRJNA73113 | ENA

Yersinia pestis biovar Orientalis str. IP275

Project description:Orientalis subtype isolated in Madagascar that causes plague

| PRJNA16376 | ENA

Yersinia pestis biovar Orientalis str. AS200901156

Project description:Genomic biosurveillance of 2009 epizootic Yersinia pestis in New Mexico, USA

| PRJNA73101 | ENA

Yersinia pestis biovar Orientalis str. AS200902147

Project description:Genomic biosurveillance of 2009 epizootic Yersinia pestis in New Mexico, USA

| PRJNA73109 | ENA

Yersinia pestis biovar Orientalis str. BA200901799

Project description:Genomic biosurveillance of 2009 epizootic Yersinia pestis in New Mexico, USA

| PRJNA72923 | ENA

Yersinia pestis biovar Antiqua str. B42003004

Project description:Antiqua biovar of the causative agent of plague

| PRJNA17685 | ENA

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data