ABSTRACT: Gene profiling of primary chicken embryo fibroblasts (CEFs) grown under the conditions of contact-inhibition, serum starvation, or both in comparison to normal cycling cells
Project description:To investigate the differences of expression patterns in primary chicken embryo fibroblasts (CEFs) under conditions of contact-inhibition and serum starvation, we undertook a gene profiling study to characterize the transcriptomes of CEFs grown under conditions of contact inhibition, serum starvation or both, in relation to normal growing (cycling) cells.
Project description:Quiescence is a tempoary arrest from the cell cycle that can be induced by many methods, including contact inhibition, fluid shear stress, and serum starvation. p27 is important to inhibit cell cycle progression. Here we evaluate transcriptional differences between three different methods of quiescence induction, flow, contact inhibition and serum starvation, in the presence or depletion of p27.
Project description:Transcription profiling from human primary fibroblasts grown either in the presence of serum (15% FBS) or arrested after serum starvation (0% FBS).
