Project description:The stability of long noncoding RNAs (lncRNAs) and mRNAs in human plasma was identified through expression profiling of genes across different time points (0, 0.5 hour, 1 hour, 2 hours, 4 hours, and 6 hours).
Project description:To investigated the therapeutic mechanism underlying the MK-626 improves vascular endothelial functionin hypertension, we performed bioinformatics analyses on the expression profiles of long noncoding RNAs (lncRNAs) and message RNAs (mRNAs) and in the aortae from Ang Ⅱ-induced hypertensive mice with or without MK-626 treatment.
Project description:To investigated the mechanism underlying the therapeutic effects of berberine in the vasculature in hypertension, we performed systematical analyses on the expression profiles of message RNAs (mRNAs) and long noncoding RNAs (lncRNAs) in the aortae from Ang Ⅱ-induced hypertensive mice with or without berberine treatment.
Project description:Long non-coding RNAs (LncRNAs) in hypertensives and their mechanisms in regulating blood pressure still remain unexplored. The aim of present study is to construct the profiles of LncRNAs in blood of patients with essential hypertension and healthy controls. Methods and results, LncRNA microarray identified up-regulated, anddown-regulated LncRNAs, in hypertensives compared to their healthy controls. Among them, one vascular smooth muscle (VSM)-specific LncRNA AK096656 (LncVSM) was quantitated in plasma of patients with hypertension and their healthy controls using the real-time qRT-PCR. LncVSM shows specific expression in human arterial vascular smooth muscle cells (HASMCs) and promote its proliferation and migration. Expression profiles and Ingenuity Pathway Analysis (IPA) revealed that LncVSM activated Renin-Angiotensin Signaling (RAS). the overexpression of LncVSM would result hypertension related complications. LncVSM (AK098656) transfection
