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Tetrahymena thermophila

ABSTRACT: mRNA sequencing of WT and cyc17∆ cells in conjugation 4, 5, 6 and 7 (C4, C5, C6 anc C7) hours of Tetrahymena thermophila

PROVIDER: PRJNA310873 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR3145130_1.fastq.gz	Fastqsanger.gz
	SRR3145130_2.fastq.gz	Fastqsanger.gz
	SRR3145131_1.fastq.gz	Fastqsanger.gz
	SRR3145131_2.fastq.gz	Fastqsanger.gz
	SRR3145132_1.fastq.gz	Fastqsanger.gz

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RNA-seq analysis of Tetrahymena thermophila

Project description:We present a comprehensive transcriptome of ciliate T. thermophila using the Illumina RNA-seq platform. The data was generated from the six mRNA samples of growth, starvation and conjugation of Tetrahymena. Despite an AT rich genome, there are about 124.6 million reads mapped to T. thermophila genome. Using these mapped reads, we have significantly improved the previous genome annotation and investigated the gene expression. Besides, our result also provided a comprehensive understanding of the alternative splicing in T. thermophila, and suggested the existence of the regulated unproductive splicing and translation (RUST) in the single-celled eukaryote. RNA-seq for six samples of Tetrahymena growth, starvation and conjugation.

2012-03-15 | E-GEOD-27971 | biostudies-arrayexpress

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Project description:mRNA sequencing of WT and e2fl-1∆ cells in conjugation 2, 3, 4, 5, 6 and 7 (C2, C3, C4, C5, C6 and C7) hours of Tetrahymena thermophila

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Tetrahymena thermophila

Project description:mRNA sequencing of WT, ∆EZL1 and ∆DCL1 cells in conjugation 3, 6 and 10 (C3, C6 anc C10) hours of Tetrahymena thermophila

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Tetrahymena thermophila

Project description:Small RNA sequencing of WT, ∆EZL1 and ∆DCL1 cells in conjugation 3, 6 and 10 (C3, C6 anc C10) hours of Tetrahymena thermophila

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Project description:Genome-Wide gene expression of Tetrahymena thermophila conjugation

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Transcriptional response of Tetrahymena thermophila to exogenous cholesterol

Project description:The model ciliate Tetrahymena thermophila satisfies its growth using triterpenoid alcohols, mainly tetrahymanol, as a surrogate of sterols. When sterols are present in the environment, T. thermophila efficiently incorporates and modifies them. T. thermophila can modify exogenous sterols by desaturation at positions C5(6), C7(8) and C22(23), and also by de-ethylation of C24. Three out of four of the enzymes involved in the sterol modification pathway were previously described by our group. However, identification of the sterol C22 desaturase remained elusive, as well as other basic aspects of this metabolism. To get more insights into this peculiar metabolism we here perform a whole transcriptome analysis of T. thermophila in response to exogenous cholesterol. We found 356 T. thermophila genes to be differentially expressed after supplementation with cholesterol for two hours. Among those genes that were upregulated under our experimental conditions, we identified two genes belonging to the long-spaced family of desaturases that we propose as putative sterol C22 desaturases. Moreover, we determined that the inhibition of tetrahymanol synthesis after sensing exogenous cholesterol occurs by a transcriptional downregulation of genes involved in squalene synthesis and cyclization. Finally, we also identified several uncharacterized genes that can be putatively involved in sterols transport and signaling.

2019-11-05 | GSE130336 | GEO

RNA-seq analysis of Tetrahymena thermophila

2012-03-15 | GSE27971 | GEO

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Project description:Analysis of conjugation-specific 26-32nt siRNAs of Tetrahymena thermophila

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Transcriptome Profile of CYC2 K.O. and WT during meiosis in Tetrahymena thermophila

Project description:Meiotic recombination is carried out through a specialized pathway for the formation and repair of DNA double-strand breaks (DSBs) made by the Spo11 protein. The present study shed light on the functional role of Cyclin, CYC2, in Tetrahymena thermophila which has transcriptionally high expression level during meiosis process. Knocking out the CYC2 gene results in arrest of meiotic conjugation process at 2.5â??3.5 h after conjugation initiation, before the meiosis division starts, and in company with the absence of DSBs. To investigate the underlying mechanism of this phenomenon, a complete transcriptome profile was performed between wild-type strain and CYC2 knock-out strain. Functional analysis of RNA-Seq results identifies related differentially expressed genes (DEGs) including SPO11 and these DEGs are enriched in DNA repair/mismatch repair (MMR) terms in homologous recombination (HR), which indicates that CYC2 could play a crucial role in meiosis by regulating SPO11 and participating in HR. We performed mRNA profiling on both wild-type strains and CYC2-knocking out strains at four different stages during meiosis prohase of Tetrahymena thermophila.

2016-04-06 | E-GEOD-79286 | biostudies-arrayexpress

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