Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Whole-transcriptome gene expression profiling in TRPC1-silenced cells

ABSTRACT: Whole-transcriptome gene expression profiling in TRPC1-silenced cells

OTHER RELATED OMICS DATASETS IN: GSE77616GSE77618

PROVIDER: PRJNA311036 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Whole-transcriptome gene expression profiling in TRPC1-silenced hepatocellular carcinoma cells.

Project description:Effects of TRPC1 silencing on whole-transcriptome gene expression were determined in Huh7 hepatocellular carcinoma cells using whole-transcriptome gene expression profiling.

2017-02-14 | GSE77386 | GEO

Whole-transcriptome gene expression profiling in TRPC1-silenced human primary aortic smooth muscle cells.

Project description:Effects of TRPC1 silencing on whole-transcriptome gene expression were determined in human primary aortic vascular smooth muscle cells using whole-transcriptome gene expression profiling.

2019-02-05 | GSE77616 | GEO

Whole-transcriptome gene expression profiling in TRPC1-silenced cells

Project description:This SuperSeries is composed of the SubSeries listed below.

2017-02-14 | GSE77618 | GEO

Gene expression in p62/SQSTM1-silenced A549 cells.

Project description:Gene expression in p62/SQSTM1-silenced A549 cells.

| PRJNA860182 | ENA

mRNA expression of Control or TRIB3-silenced MCF7 cells

Project description:Investigation of whole genome gene expression level changes in TRIB3-silenced MCF7 cells as compared to Control MCF7 cells. Analysis of activity changes of pathways for FOXO1 phosphorylation between TRIB3-silenced and Control MCF7 cells.

2019-09-25 | GSE116671 | GEO

Differential gene expression in UCHL1 silenced ovarian cancer cells

Project description:Differential gene expression in UCHL1 silenced ovarian cancer cells

| PRJNA714628 | ENA

Whole transcriptome amplification for gene expression profiling and development of molecular archives.

Project description:Expression profiling of clinically obtainable tumor specimens has been hindered by the need for microgram quantities of RNA. In vitro transcription (IVT)-based amplifications are most commonly used to amplify small quantities of RNA for microarray analysis. However, significant drawbacks exist with IVT-based amplification, and the need for alternative amplification methods remains. Herein, we validate whole transcriptome amplification (WTA), an exponential amplification technique that produces cDNA libraries and amplified target in 3 to 4 hours from nanogram quantities of total RNA using a combination of cDNA microarrays and quantitative polymerase chain reaction (PCR). We demonstrate that WTA material can serve as a "molecular archive" because a WTA cDNA library can be faithfully amplified through multiple rounds of PCR amplification, allowing it to serve as a bankable and distributable resource. To demonstrate applicability, WTA was combined with laser capture microdissection to profile frozen prostate tissues. Unlike most IVT-based and exponential amplification techniques, WTA does not depend on the presence of a poly-A tail. Thus, we demonstrate that WTA is compatible with artificially degraded RNA and RNA isolated from formalin-fixed paraffin-embedded tissues. Taken together, WTA represents a versatile approach to profile and archive cDNA from minute tumor samples and is compatible with partially degraded RNA.

| S-EPMC1578511 | biostudies-literature

Whole transcriptome profiling of taste bud cells.

Project description:Analysis of single-cell RNA-Seq data can provide insights into the specific functions of individual cell types that compose complex tissues. Here, we examined gene expression in two distinct subpopulations of mouse taste cells: Tas1r3-expressing type II cells and physiologically identified type III cells. Our RNA-Seq libraries met high quality control standards and accurately captured differential expression of marker genes for type II (e.g. the Tas1r genes, Plcb2, Trpm5) and type III (e.g. Pkd2l1, Ncam, Snap25) taste cells. Bioinformatics analysis showed that genes regulating responses to stimuli were up-regulated in type II cells, while pathways related to neuronal function were up-regulated in type III cells. We also identified highly expressed genes and pathways associated with chemotaxis and axon guidance, providing new insights into the mechanisms underlying integration of new taste cells into the taste bud. We validated our results by immunohistochemically confirming expression of selected genes encoding synaptic (Cplx2 and Pclo) and semaphorin signalling pathway (Crmp2, PlexinB1, Fes and Sema4a) components. The approach described here could provide a comprehensive map of gene expression for all taste cell subpopulations and will be particularly relevant for cell types in taste buds and other tissues that can be identified only by physiological methods.

| S-EPMC5548921 | biostudies-literature

Gene expression in p62/SQSTM1-silenced A549 cells.

Project description:We used microarrays to detail the programme of gene expression in control or p62/SQSTM1-silenced A549 cells.

2022-07-21 | GSE208588 | GEO

GATA4 silenced HUH6 hepatoblastoma cells

Project description:GATA4 silenced HUH6 hepatoblastoma cells

| PRJNA434561 | ENA