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Whole-transcriptome gene expression profiling in TRPC1-silenced cells

ABSTRACT: Whole-transcriptome gene expression profiling in TRPC1-silenced cells

OTHER RELATED OMICS DATASETS IN: GSE77616GSE77618

PROVIDER: PRJNA311036 | ENA |

REPOSITORIES: ENA

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Whole-transcriptome gene expression profiling in TRPC1-silenced hepatocellular carcinoma cells.

Project description:Effects of TRPC1 silencing on whole-transcriptome gene expression were determined in Huh7 hepatocellular carcinoma cells using whole-transcriptome gene expression profiling.

2017-02-14 | GSE77386 | GEO

Whole-transcriptome gene expression profiling in TRPC1-silenced human primary aortic smooth muscle cells.

Project description:Effects of TRPC1 silencing on whole-transcriptome gene expression were determined in human primary aortic vascular smooth muscle cells using whole-transcriptome gene expression profiling.

2019-02-05 | GSE77616 | GEO

Whole-transcriptome gene expression profiling in TRPC1-silenced cells

Project description:This SuperSeries is composed of the SubSeries listed below.

2017-02-14 | GSE77618 | GEO

Function of the porcine TRPC1 gene in myogenesis and muscle growth

Project description:Muscle growth is a quantitative trait controlled by multiple genes in animals. Previously, we showed that transient receptor potential channel 1 (TRPC1) was a differentially expressed gene in muscle tissues between pig breeds with divergent growth traits using RNA-seq. In this study, we characterized the expression profiles of TRPC1 in different tissues and various pig breeds, and found that TRPC1 is highly expressed in muscle. We found two effective single nucleotide polymorphism (SNP) sites (C-1763T and C-1604T) in TRPC1 that could affect the activity of the promoter region and regulate the growth rate of pigs. Functionally, we used RNAi and overexpression to illustrate that TRPC1 promotes myoblast proliferation, migration, differentiation, fusion, and muscle hypertrophy, while inhibiting muscle degradation, and that these processes may be mediated by activating the Wnt signaling pathways. Taken together, our results revealed that TRPC1 may be a promoter of muscle growth and development and it plays a role in Wnt-mediated myogenesis.

2020-11-07 | GSE160972 | GEO

Effect of Trpc1 or Trpc6 knockout on gene expressions in the hearts of endotoxemic mice

Project description:To investigate the mechanism of TRPC1 or TRPC6 on the regulation of endotoxemic cardiac dysfunction, we established Trpc1-/- and Trpc6-/- mice. LPS-challenged endotoxemic mouse model was built, and the gene expression profile was analyzed using data obtained from RNA-seq of WT, LPS-challenged WT, LPS-challenged Trpc1-/-, and LPS-challenged Trpc6-/- mice hearts.

2022-11-06 | GSE217156 | GEO

TRPC1 links calcium signaling to cellular senescence in the protection against post-traumatic osteoarthritis

Project description:Transient receptor potential channel 1 (TRPC1) is a widely expressed mechanosensitive ion channel located within the endoplasmic reticulum membrane, crucial for refilling depleted internal calcium stores during activation of calcium-dependent signaling pathways. Here, we have demonstrated that TRPC1 activity is protective within cartilage homeostasis in the prevention of cellular senescence associated cartilage breakdown during mechanical and inflammatory challenge. We revealed that TRPC1 loss is associated with early stages of osteoarthritis (OA) and plays a non-redundant role in calcium signaling in chondrocytes. Trpc1-/- mice subjected to destabilization of the medial meniscus induced OA developed a more severe OA phenotype than wild type controls. During early OA development, Trpc1-/- mice displayed an increased chondrocyte survival rate, however remaining cells displayed features of senescence including p16INK4a expression and decreased Sox9. RNA sequencing identified differentially expressed genes related to cell number, apoptosis and extracellular matrix organization. Trpc1-/- chondrocytes exhibited accelerated dedifferentiation, while demonstrating an increased susceptibility to cellular senescence. Targeting the mechanism of TRPC1 activation may be a promising therapeutic strategy in osteoarthritis prevention.

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Whole transcriptome amplification for gene expression profiling and development of molecular archives.

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mRNA expression of Control or TRIB3-silenced MCF7 cells

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Evaluation of bovine embryo quality based on gene expression profiling using whole-transcriptome amplification.

Project description:This study aimed to develop a method to evaluate the quality of bovine in vitro fertilized (IVF) embryos based on gene expression profiling via whole-transcriptome amplification. The expression of 11 developmentally important genes in individual bovine in vivo-derived (IVD) and IVF embryos were examined. Gene expression profiling was conducted by classifying the expression level of each gene in individual embryos as low, medium, or high. The IVF group had a higher (P < 0.01) proportion of embryos with low expression of SOX2, NANOG, and FGF4. In addition, a correlation analysis between the expression levels of each gene in individual embryos demonstrated that the relationship between gene expression differed with respect to IVD and IVF embryos. Our results suggest that the expression profiling of developmentally important genes using IVD embryos as normal controls could be a useful indicator for evaluating the quality of bovine IVF embryos.

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Gene expression in p62/SQSTM1-silenced A549 cells.

Project description:Gene expression in p62/SQSTM1-silenced A549 cells.

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