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Homo sapiens

ABSTRACT: Cell freezing protocol suitable for ATAC-Seq on motor neurons derived from human induced pluripotent stem cells

PROVIDER: PRJNA312393 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR3176243.fastq.gz	Fastqsanger.gz
	SRR3176244.fastq.gz	Fastqsanger.gz
	SRR3176245.fastq.gz	Fastqsanger.gz
	SRR3176246.fastq.gz	Fastqsanger.gz
	SRR3176247.fastq.gz	Fastqsanger.gz

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Cell freezing protocol suitable for ATAC-Seq on motor neurons derived from human induced pluripotent stem cells

Project description:We describe a protocol for freezing neuronal cells that is compatible with ATAC-Seq, producing results that compare well with those generated from fresh cells. We developed our protocol on a disease-relevant cell type, namely motor neurons differentiated from induced pluripotent stem cells from a patient affected by spinal muscular atrophy. We found that while flash-frozen motor neurons are not suitable for ATAC-Seq, the assay is successful with slow-cooled cryopreserved samples. Using this method, we were able to isolate high quality, intact nuclei, and we verified that epigenetic results from fresh and cryopreserved motor neurons agree quantitatively.

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