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Ictalurus punctatus

ABSTRACT: More than Just Antibodies: Protective Mechanisms of a Mucosal Vaccine against Fish Pathogen Flavobacterium Columnare

PROVIDER: PRJNA312527 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR3195330_1.fastq.gz	Fastqsanger.gz
	SRR3195330_2.fastq.gz	Fastqsanger.gz
	SRR3195350_1.fastq.gz	Fastqsanger.gz
	SRR3195350_2.fastq.gz	Fastqsanger.gz
	SRR3195342_1.fastq.gz	Fastqsanger.gz

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Ictalurus punctatus

Project description:Early Divergent Responses to Virulent and Attenuated Vaccine Isolates of Flavobacterium columnare in Channel Catfish, Ictalurus punctatus

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Gene expression profiles of white bass (Morone chrysops) and hybrid striped bass (M. chrysops x M. saxatilis) gill tissue over time following Flavobacterium covae exposure

Project description:Columnaris disease is a prevalent disease in freshwater environments worldwide caused by the ubiquitous aquatic bacterium Flavobacterium species. Adhesion to the external mucosal surfaces of fishes is the initial stage of infection, and the gills specifically have been identified as both a primary target and release site for this pathogen. Previous research has indicated that a predominant US aquaculture product, the hybrid striped bass (Morone chrysops x M. saxatilis), is more susceptible to infection with Flavobacterium columnare (covae) than the maternal white bass (M. chrysops) parental species. Therefore, to further elucidate the differences between these fish we conducted a transcriptomic profiling study examining the differences of gene expression in gill mucosal tissue over time after exposure to F. covae isolate LSU-066-04. Combined with previous work, these data provide a greater understanding of host immune response to a common pathogen in moronids.

2024-05-09 | GSE246056 | GEO

Gene expression profiling of catfish after treatment with Flavobacterium columnare DnaK protein

Project description:Catfish farming remains number one among U.S. aquaculture production. Flavobacterium columnare, the causative agent of columnaris disease, produces substantial mortality during the production of freshwater farmed finfish species. F. columnare is ubiquitous in the aquatic environment, and outbreaks of disease are often triggered during the spring and summer months of the catfish production cycle. As food fish production continues to increase, the frequency of columnaris disease will only continue to rise within the aquaculture industry and thus new preventative measures will be required. Vaccination against different fish pathogens has proven to be an effective disease management practice within the aquaculture industry. To evaluate the immunological effect of bath immunization on catfish with a recombinant F. columnare DNAk protein vaccine, we performed RNA sequencing on skin samples from control and rDNAK immunized catfish at different time intervals. We evaluated the different gene expression patterns between the groups with a focus on identifying differences in innate and adaptive immune function.

2019-06-20 | GSE121116 | GEO

Ictalurus punctatus

Project description:The channel catfish is an important commercial fish

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Ictalurus punctatus

Project description:Evasion of Mucosal Defenses During Aeromonas hydrophila Infection of Channel Catfish (Ictalurus puncatus) Skin

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Ictalurus punctatus

Project description:Impact of feed additives on surface mucosal health and columnaris susceptibility in channel catfish fingerlings

| PRJNA277577 | ENA

Ictalurus punctatus

Project description:Impact of feed additives on surface mucosal health and columnaris susceptibility in channel catfish fingerlings (ictalurus punctatus)

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Proteomic analysis of the fish pathogen Flavobacterium columnare

Project description:Clinical Flavobacterium columnare ATCC 49512 was grown on Flavobacterium columnare growth medium (FCGM). Bacteria from four colonies at mid-exponential phase were harvested, total proteins were isolated, and identified using 2-DE MALDI TOF/TOF MS and 2-D LC ESI MS/MS analyses. The MS/MS spectra for all peptides were analyzed using sequest algorithm

2016-03-11 | PRD000810 | Pride

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Project description:Expression analysis of channel catfish anterior kidney at 48h post immersion challenge of vaccinated fish (28 days post vaccination with attenuated Edwardsiella ictaluri) with virulent E. ictaluri

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Gene expression analysis between planktonic and biofilm states of Flavobacterium columnare

Project description:Flavobacterium columnare, the causative agent of columnaris disease causes substantial mortality worldwide in numerous freshwater finfish species. Due to its global significance and impact on the aquaculture industry continual efforts to better understand basic mechanisms that contribute to disease are urgently needed. F. columnare naturally occurs in a planktonic, free living state where it can survive for long periods of time, even in the absence of nutrients. In contrast, F. columnare also possesses the ability to form biofilms, broadly defined as surface bound microbial communities inhabiting an organic matrix composed of autogenously derived extracellular polymeric substances. The advantages of adopting this life stage are not completely clear for F. columnare, but biofilm formation could increase virulence by offering protection from desiccation, augment resistance to antimicrobials, improve nutrient acquisition, and protection against other bacteria. To examine gene expression between F. columnare planktonic cells and biofilms, we conducted a study where both phases were grown with and without stimulation and then sampled for RNA sequencing.

2018-10-16 | GSE109937 | GEO

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