Project description:To investigate the genome-wide effects of ica transgenic line overexpressing AT5G40830 (ICA) involved in the cambial activity and shoot growth, we used cDNA microarrays to establish global changes in the gene expression. We selected the uppermost 1 cm of vegetative nodes in the inflorescence stems at the sixth silique stage. These showed the most distinctive changes in phenotypes and endogenous hormone levels between ica and Col-0. Expression of 9 genes (ARR5, ARR6, ARR7, EXP1, GASA4, GASA6, PME1, PME44, AT3G49220) based on microarray analysis was quantified by real-time PCR.
Project description:Transcriptional profiling of Arabidopsis rossette leaves comparing WT Col-0 with a transgenic line overexpressing AhERF or AhDOF genes from Amaranthus hypochondriacus under different conditions.
Project description:Transcriptional profiling of Arabidopsis rossette leaves comparing WT Col-0 with a transgenic line overexpressing AhDGR gene from Amaranthus hypochondriacus.
Project description:Transcriptional profiling of Arabidopsis rossette leaves comparing WT Col-0 with a transgenic line overexpressing Ah24 gene from Amaranthus hypochondriacus.
Project description:CuZn-superoxide dismutase (CuZn-SOD) and ascorbate peroxidase (APX) constitute first line of defence against oxidative stress. In the present study, PaSOD and RaAPX genes from Potentilla atrosanguinea and Rheum australe, respectively were overexpressed individually as well as in combination in Arabidopsis thaliana. We performed RNA-seq analysis of wild type and transgenic Arabidopsis thaliana overexpressing CuZn-SOD, APX and CuZn-SOD + APX under control and salt stress
