Project description:Transcriptional profiling of salivary gland, midgut and ovary tissues isolated from Rhipicephalus microplus females at day 20 post infestation. This enabled the identification of transcripts that are tissue-specific or shared among the tissues tested. Reference pool design: Each tissue tested was compared to a reference pool comprising ticks (immature to adult stages) sampled on day 4, 5, 7, 13, 15 and tissues collected on day 20 post infestation. Biological replicates: 2; Technical replicates: 2.

2013-03-15 | E-GEOD-35867 | biostudies-arrayexpress

Comparative microarray analysis of midgut tissues from feeding Rhipicephalus microplus and Rhipicephalus decoloratus adult females

2015-01-31 | GSE55826 | GEO

Microarray analysis of gene expression in acaricide exposed Rhipicephalus (Boophilus) microplus.

Project description:A R. microplus microarray was used to study differential gene expression in acaricide exposed larvae from an amitraz-resistant strain. The acaricide treatments were: organophosphate (OP), pyrethroid, ivermectin, and amitraz. The microarrays contained over 13,000 probes corresponding to each member of R. microplus gene index ESTs previously described (http://compbio.dfci.harvard.edu/tgi/cgi-bin/tgi/gimain.pl?gudb=b_microplus). Serial analysis of gene expression (SAGE) data from the OP treated R. microplus was used to verify the OP microarray data. The expression profiles of selected transcripts were verified by real time PCR. Among the significantly differentially expressed genes, were a tick legumain, involved in blood digestion, gluthathione S-transferase (GST), a detoxification enzyme involved in pesticide resistance, acyltransferase, several putative salivary sulfotransferases, and a glutamate receptor. **Note: contact person: Felix D. Guerrero email: felix.guerrero@ars.usda.gov Keywords: Rhipicephalus (Boophilus) microplus, acaricide resistance genes, organophosphates OP, microarrays, detoxification enzymes.

2008-12-31 | GSE10171 | GEO

Rhipicephalus microplus

Project description:Rhipicephalus microplus overview

| PRJNA46693 | ENA

Rhipicephalus microplus

Project description:Rhipicephalus microplus Transcriptome or Gene expression

| PRJNA82295 | ENA

Rhipicephalus microplus

Project description:Rhipicephalus microplus Genome sequencing

| PRJNA1052456 | ENA

Comparative microarray analysis of midgut tissues from feeding Rhipicephalus microplus and Rhipicephalus decoloratus adult females

Project description:Transcriptional profiling of midgut tissues isolated from Rhipicephalus microplus and Rhipicephalus decoloratus females at day 20 post infestation. This enabled the identification of transcripts that are species-specific or shared between the two tick species tested. A direct comparison (Balanced block) was performed: Midgut tissues collected on day 20 (post infestation) from R. microplus was compared to that of R. decoloratus. Biological replicates: 3 ; Technical replicates: 1.

2015-01-31 | E-GEOD-55826 | biostudies-arrayexpress

Rhipicephalus microplus

Project description:Rhipicephalus microplus Raw sequence reads

| PRJNA1028823 | ENA