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Interference with ethylene perception at receptor level sheds light on auxin and transcriptional circuits associated with climacteric ripening of apple fruit (Malus x domestica Borkh.)

ABSTRACT: Interference with ethylene perception at receptor level sheds light on auxin and transcriptional circuits associated with climacteric ripening of apple fruit (Malus x domestica Borkh.)

PROVIDER: PRJNA313420 | ENA |

REPOSITORIES: ENA

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Interference with ethylene perception at receptor level sheds light on auxin and transcriptional circuits associated with climacteric ripening of apple fruit (Malus x domestica Borkh.)

Project description:Apple (Malus x domestica Borkh.) is a model fruit species to study the metabolic changes occurring at the onset of ripening as well the physiological mechanism governed by the hormone ethylene. In this survey, to dissect the climacteric interplay in apple, a multidisciplinary approach was employed. To this end, a comprehensive analysis of gene expression together with the investigation of several physiological entities (texture, volatilome and polyphenolic compounds) was carried out throughout fruit development and ripening. The transcriptomic profiling was conducted with two microarray platforms, a custom array dedicated to fruit ripening pathways (iRIPE) and a whole genome array specifically enriched of ripening related genes for apple (WGAA). The transcriptomic and phenotypic changes following the application of 1-methylcyclopropene (1-MCP), an ethylene inhibitor, were also highlighted. The suppression of ethylene modified and delayed the ethylene receptors turnover, leading to important modifications in the overall fruit physiology. The integrative comparative network analysis showed both negative and positive correlations between ripening related transcripts and accumulation of specific metabolites or texture components. The ripening distortion caused by the inhibition of the ethylene perception besides affecting the ethylene and texture control, stimulated the de-repression of auxin related genes, transcription factors and photosynthethic genes. In the end, the comprehensive repertoire of results obtained here step forwards in the elucidation of the multi-layered control of ethylene, hypothesizing a possible hormonal cross-talk coupled with a transcriptional regulation. 48 samples analyzed; 8 stages have been identified over the fruit development and ripening (from flower to post harvest ripening) of apple fruit belonging to two apple cultivars (Golden Delicious and Granny Smith), ending with 16 samples (3 replacates for each sample)

2016-08-23 | E-GEOD-78745 | biostudies-arrayexpress

Interference with ethylene perception at receptor level sheds light on auxin and transcriptional circuits associated with climacteric ripening of apple fruit (Malus x domestica Borkh.)

2016-08-23 | GSE78745 | GEO

Interference with ethylene perception at receptor level sheds light on auxin and transcriptional circuits associated with the climacteric ripening of apple fruit (Malus x domestica Borkh.)

2016-08-23 | GSE78744 | GEO

Interference with ethylene perception at receptor level sheds light on auxin and transcriptional circuits associated with climacteric ripening of apple fruit (Malus x domestica Borkh.)

Project description:This SuperSeries is composed of the SubSeries listed below.

2016-08-23 | GSE78746 | GEO

Interference with ethylene perception at receptor level sheds light on auxin and transcriptional circuits associated with climacteric ripening of apple fruit (Malus x domestica Borkh.)

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2016-08-23 | E-GEOD-78746 | biostudies-arrayexpress

Integrative approach combining transcriptomic assay with physical and metabolite analysis reveal novel clues about the regulatory mechanism governing the climacteric ripening physiology in apple (Malus x domestica Borkh.) [WGAA]

Project description:Apple (Malus x domestica Borkh.) is a model fruit species to study the metabolic changes occurring at the onset of ripening as well the physiological mechanism governed by the hormone ethylene. In this survey, to dissect the climacteric interplay in apple, a multidisciplinary approach was employed. To this end, a comprehensive analysis of gene expression together with the investigation of several physiological entities (texture, volatilome and polyphenolic compounds) was carried out throughout fruit development and ripening. The transcriptomic profiling was conducted with two microarray platforms, a custom array dedicated to fruit ripening pathways (iRIPE) and a whole genome array specifically enriched of ripening related genes for apple (WGAA). The transcriptomic and phenotypic changes following the application of 1-methylcyclopropene (1-MCP), an ethylene inhibitor, were also highlighted. The suppression of ethylene modified and delayed the ethylene receptors turnover, leading to important modifications in the overall fruit physiology. The integrative comparative network analysis showed both negative and positive correlations between ripening related transcripts and accumulation of specific metabolites or texture components. The ripening distortion caused by the inhibition of the ethylene perception besides affecting the ethylene and texture control, stimulated the de-repression of auxin related genes, transcription factors and photosynthethic genes. In the end, the comprehensive repertoire of results obtained here step forwards in the elucidation of the multi-layered control of ethylene, hypothesizing a possible hormonal cross-talk coupled with a transcriptional regulation. whole genome array specifically enriched of ripening related genes for apple (WGAA) with two cultivars (Golden Delicious and Granny Smith)

2016-08-23 | E-GEOD-78744 | biostudies-arrayexpress

The ethylene-auxin crosstalk regulates the postharvest fruit ripening process in apple

Project description:The ripening of climacteric fruits, such as apple, is represented by a series of genetically programmed events orchestrated by the action of several hormones. In this work, we investigated the existence of a hormonal crosstalk between ethylene and auxin during the post-harvest ripening of three internationally known apple cultivars: ‘Golden Deli-cious’, ‘Granny Smith’ and ‘Fuji’. The normal climacteric ripening was impaired by the exogenous application of 1-methylcyclopropene (1-MCP) that effectively affected the production of ethylene and the physiological behaviour of specific ethylene-related qual-ity traits, such as fruit texture and the production of volatile organic compounds showed a de-novo accumulation of auxin following the application of 1-MCP. The RNA-Seq wide-transcriptome analysis evidenced as the competition at the level of the ethylene re-ceptors induced a cultivar-dependent transcription re-programming. The DEGs annota-tion carried out through the KEGG database identified as most genes were assigned to the plant hormone signaling transduction category, and specifically related to auxin and ethylene. The interplay between these two hormones was further assessed through a candidate gene analysis that highlighted a specific activation of GH3 and ILL genes, en-coding key steps in the process of the auxin homeostasis mechanism. Our results showed that a compromised ethylene metabolism at the onset of the climacteric ripening in apple can stimulate, in a cultivar-dependent fashion, an initial de-novo synthesis and de-conjugation of auxin as a tentative to restore a normal ripening progression.

2021-11-17 | GSE182822 | GEO

1-MCP effect on the transcriptional profiling of apple flesh (Malus x domestica Borkh, Gala variety) maintained in cold storage

Project description:Apples (Malus x domestica Borkh, Gala variety) grown in commercial orchards were harvested at maturity and maintained 2 months in cold storage with or without 1-MCP treatment.

2017-08-28 | GSE59728 | GEO

Transcriptional profiling of Granny Smith apple peel (Malus x domestica Borkh) at harvest between contrasted years for scald incidence

Project description:Granny Smith apples (Malus x domestica Borkh) were harvested at early physiological mature stage in 2014, 2015 and 2017 and phenotyped for scald incidence after long time cold storage. Differential analyses were set-up for peel samples collected at harvest between fruit batches with low or high scald incidence.

2020-04-20 | GSE135863 | GEO

Effect of 1-MCP and ozone post-harvest treatments on the transcriptional profiling of Granny Smith apple peel (Malus x domestica Borkh) after cold storage.

Project description:Granny Smith apples (Malus x domestica Borkh) grown in commercial orchards were harvested at physiological mature stage and maintained 6 months in cold storage with or without 1-MCP post-harvest treatment, and with or without enriched ozone atmosphere.

2020-03-09 | GSE133456 | GEO

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