Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Soil metagenome

ABSTRACT: Soil sample of tomato and cucumber plants Raw sequence reads

PROVIDER: PRJNA313501 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR3195595.fastq.gz	Fastqsanger.gz
	SRR3195595_1.fastq.gz	Fastqsanger.gz
	SRR3195595_2.fastq.gz	Fastqsanger.gz

Items per page:

1 - 3 of 3

Similar Datasets

soil metagenome

Project description:Soil sample of cucumber Raw sequence reads

| PRJNA305468 | ENA

soil metagenome

Project description:Soil sample of tomato Raw sequence reads

| PRJNA313500 | ENA

soil metagenome

Project description:Soil sample of cucumber plant Raw sequence reads

| PRJNA305477 | ENA

soil metagenome

Project description:Soil sample of tomato plant Raw sequence reads

| PRJNA313499 | ENA

soil metagenome

Project description:The soil sample of tomato plant Raw sequence reads

| PRJNA305179 | ENA

Next Generation Sequencing Facilitates Quantitative Analysis of Cucumber and Botrytis cinerea Transcriptome Changes During Infection

Project description:Purpose: The goals of this study are using RNA-seq to obtain cucumber and Botrytis cinerea transcriptome changes during infection Methods: mRNA profiles of anti-infection samples and interaction sample were generate by deep sequencing,using Illumina Hiseq 2500. The sequence reads that passed quality filters were analyzed at the transcript isoform level with two methods: Burrows–Wheeler Aligner (BWA) followed by ANOVA (ANOVA) and TopHat followed by Cufflinks. qRT–PCR validation was performed using SYBR Green assays Results: Using an optimized data analysis workflow,In total, 248,908,688 raw reads were generated; after removing low-quality reads and those containing adapter and poly-N, 238,341,648 clean reads remained to map the reference genome. There were 3,512 cucumber (differential expression genes) DEGs and 1,735 B. cinerea DEGs. GO enrichment and KEGG enrichment analysis were performed on these DEGs to study the interaction between cucumber and B. cinerea. To verify the reliability and accuracy of our transcriptome data, 5 cucumber DEGs and 5 B. cinerea DEGs were chosen for RT-PCR verification. Conclusions:To the best of our knowledge, this is the first analysis of large-scale transcriptome changes of cucumber during the infection of Botrytis cinerea. These results will increase our understanding of the molecular mechanisms of the cucumber defense Botrytis cinerea and may be used to protect plants against disasters caused by necrotrophic fungal pathogens.

2016-08-01 | GSE72191 | GEO

soil metagenome

Project description:soil sample Raw sequence reads

| PRJNA356329 | ENA

soil metagenome

Project description:soil sample Raw sequence reads

| PRJNA386097 | ENA

soil metagenome

Project description:soil sample Raw sequence reads

| PRJNA386598 | ENA

soil metagenome

Project description:soil sample Raw sequence reads