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CEL-Seq2: sensitive highly-multiplexed single-cell RNA-Seq

ABSTRACT: CEL-Seq2: sensitive highly-multiplexed single-cell RNA-Seq

OTHER RELATED OMICS DATASETS IN: GSE78779

PROVIDER: PRJNA313513 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR3195900.fastq.gz	Fastqsanger.gz
	SRR3195910.fastq.gz	Fastqsanger.gz
	SRR3195920.fastq.gz	Fastqsanger.gz
	SRR3195930.fastq.gz	Fastqsanger.gz
	SRR3195940.fastq.gz	Fastqsanger.gz

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CEL-Seq2: sensitive highly-multiplexed single-cell RNA-Seq

Project description:Single-cell transcriptomics requires a method that is sensitive, accurate, and reproducible. Here, we present CEL-Seq2, a modified version of our CEL-Seq method, with three-fold higher sensitivity, lower costs, and less hands-on time. We also implemented CEL-Seq2 on Fluidigm’s C1 system, thereby providing its first single-cell, on-chip barcoding method, and detected gene expression changes accompanying the progression through the cell cycle in mouse fibroblast cells. We also compare with Smart-Seq to demonstrate CEL-Seq2’s increased sensitivity relative to other available methods. Collectively, the improvements make CEL-Seq2 uniquely suited to single-cell RNA-Seq analysis in terms of economics, resolution, and ease of use

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